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Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3
From 2014.igem.org
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Revision as of 14:50, 17 October 2014
Notebook
Lab Documents
H-Stem Vector Exchange to pSB1A3
-
Start
-
mini-Prep
1A3 Vector -
PCR
Plac-stem-as-stem-dT on 1C3 with 100up_F & 200dn_R -
Digestion
1A3 with EcoRI & PstI, Plac-stem-as-stem-dT with EcoRI & PstI -
Ligation
1A3 & Plac-stem-as-stem-dT -
Transformation
Plac-stem-as-stem-dT on 1A3 -
Complete!
