Team:HokkaidoU Japan/Notebook/asB0034/asB0034-construction

(Difference between revisions)

Revision as of 14:37, 17 October 2014

hogehoge

Start
Primer Annealing
- Sun Aug 17
asB0034_F Tm: 68.7℃ asB0034_R Tm: 68.7℃
Digestion
- Sun Aug 17
Cut pHN1257 with NcoI, XhoI(using 10×cut smart)
Gel extraction
- Mon Aug 18
digested pHN1257
Ethanol Precipetation
- Mon Aug 18
digested pHN1257
Ligation
- Mon Aug 18
Ligate pHN1257 with asB0034
Transformation
- Tue Aug 19
Ptrc-stem-asB0034-stem(pHN1257) on ice 30min each of 5.0µL DNA to DH5α 42℃ 1min on ice 2min
Colony PCR
- Wed Aug 20
Ptrc-stem-asB0034-stem(pHN1257) pHN1257_up_F Tm:60.1℃ asB0034_R Tm:68.7℃
Culture
- Thu Aug 21
Ptrc-stem-asB0034-stem
mini-Prep
- Fri Aug 22
Ptrc-stem-asB0034-stem
Transformation
- Fri Aug 22
Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice 30min each of 2.5µL DNA to DH5α 42℃ 30sec on ice 2min
Complete!

@@ Line 129: / Line 129: @@
 	      </ul>
 	      <div class="step-recipe">
-asB0034-f Tm: 68.7&deg;C asB0034-r Tm: 68.7&deg;C
+asB0034_F Tm: 68.7&#8451; asB0034_R Tm: 68.7&#8451;
 </div>
 	    </div>
@@ Line 234: / Line 234: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptrc-stem-asB0034-stem(pHN1257) on ice 		30min each of 5.0&micro;L DNA to DH5&alpha; 42&deg;C        1min on ice		2min
+Ptrc-stem-asB0034-stem(pHN1257) on ice 		30min each of 5.0&micro;L DNA to DH5&alpha; 42&#8451;        1min on ice		2min
 </div>
 	    </div>
@@ Line 255: / Line 255: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptrc-stem-asB0034-stem(pHN1257) pHN1257_up_F	Tm:60.1 asB0034-R	Tm:68.7
+Ptrc-stem-asB0034-stem(pHN1257) pHN1257_up_F	Tm:60.1&#8451; asB0034_R	Tm:68.7&#8451;
 </div>
 	    </div>
@@ Line 318: / Line 318: @@
 	      </ul>
 	      <div class="step-recipe">
-Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice  30min each of 2.5&micro;L DNA to DH5&alpha; 42C     30sec on ice  2min
+Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice  30min each of 2.5&micro;L DNA to DH5&alpha; 42&#8451;     30sec on ice  2min
 </div>
 	    </div>