"
Team:LIKA-CESAR-Brasil/Hybridization
From 2014.igem.org
(Difference between revisions)
Villaverde (Talk | contribs)
(Created page with "{{CSS/Main}} <html lang="pt-br"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-wid...")
Newer edit →
(Created page with "{{CSS/Main}} <html lang="pt-br"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-wid...")
Newer edit →
Revision as of 13:53, 17 October 2014
NOTEBOOK
Hybridization Process
To perform the hybridization process, 3μL target synthetic solution (miRNA) at different concentrations (1x10-6 the 1x10-12M) was dropped on the working electrode. The electrode remained under 300 rpm agitation at a temperature of annealing of 52 ° C for 10 minutes (the description of the manufacturing company sequence). Tests positive for breast cancer provided by the Baron of Lucena-PE Hospital biological samples were also performed. After completion of the hybridization tests with different targets, hybridization tests with non-complementary targets were made.
