"
Team:Bordeaux/Parts/Assembly improvement
From 2014.igem.org
(Difference between revisions)
m (Created page with "{{:Team:Bordeaux/Tete}} We had the idea to add a new restriction site compatible with XbaI before the stop codon. This restriction site is NheI and allowed us to produce a fused...") |
m |
||
| Line 2: | Line 2: | ||
We had the idea to add a new restriction site compatible with XbaI before the stop codon. This restriction site is NheI and allowed us to produce a fused protein if the 2 inserts were cloned together following this protocol: | We had the idea to add a new restriction site compatible with XbaI before the stop codon. This restriction site is NheI and allowed us to produce a fused protein if the 2 inserts were cloned together following this protocol: | ||
| - | + | ||
| + | [[File:Bdx2014 Protein fusion.png|750px]] | ||
| + | |||
# Other advantages of NheI: | # Other advantages of NheI: | ||
#* heated-deactivation | #* heated-deactivation | ||
Revision as of 11:39, 17 October 2014
We had the idea to add a new restriction site compatible with XbaI before the stop codon. This restriction site is NheI and allowed us to produce a fused protein if the 2 inserts were cloned together following this protocol:
- Other advantages of NheI:
- heated-deactivation
- compatibility with most of the others Biobricks (RFC10, 21, 23, 25, 1000)
- obtaining a fused protein and allowing to choose the order of the genes
- Disadvantages of NheI:
- Not compatible with RFC12 standard
