Team:Bordeaux/Parts/Assembly improvement

From 2014.igem.org

We had the idea to add a new restriction site compatible with XbaI before the stop codon. This restriction site is NheI and allowed us to produce a fused protein if the 2 inserts were cloned together following this protocol:

Bdx2014 Protein fusion.png

  1. Other advantages of NheI:
    • heated-deactivation
    • compatibility with most of the others Biobricks (RFC10, 21, 23, 25, 1000)
    • obtaining a fused protein and allowing to choose the order of the genes
  2. Disadvantages of NheI:
    • Not compatible with RFC12 standard



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