Team:Groningen/Template/MODULE/projects/toolbox/gfp

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Fluorescent marker
 
Last but not least, a fluorescent marker that has a high performance in L. lactis was BioBricked and added to the toolbox. This marker is a version of the sfGFP protein, called sfGFP(Bs), that was optimized for Bacillus subtilis. When the optimized sfGFP was tested, it showed high performance in L. lactis.5 The sfGFP(Bs) can be used for the testing of constructs, quantification of promoters or it can be coupled to other proteins to track them inside or outside the cell.