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Team:Groningen/Template/MODULE/projects/toolbox/gfp
From 2014.igem.org
Fluorescent marker
Last but not least, a fluorescent marker that has a high performance in
L. lactis was BioBricked and added to the toolbox. This marker
is a version of the sfGFP protein, called sfGFP(Bs), that was optimized for Bacillus subtilis.
When the optimized sfGFP was tested, it showed high performance in
L. lactis.5 The sfGFP(Bs) can be used for the testing of
constructs, quantification of promoters or it can be coupled to other
proteins to track them inside or outside the cell.
Figure 2:
E. coli expressing sfGFP(Bs), combined with the promoters CP41 and CP44.
