Team:Caltech/Notebook

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<h1> Project Overview </h1>
<h1> Project Overview </h1>
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<p>Our project was split into 3 subprojects: AgrBDCA signal reception, FsrABC signal reception, and Ligand export systems.</p>
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<p>Our project was split into 3 subprojects: AgrBDCA signal reception & Combinatorial Promoters, FsrABC/lamBCDA signal reception, and Ligand export systems.</p>
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<h4>AgrBDCA Signal Reception</h4>
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<h4>AgrBDCA Signal Reception & Combinatorial Promoters</h4>
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AgrBDCA is a quorum sensing system found to regulate virulence factors in Staphylococcus Aurea. (sp?) In this system, agrC and agrA function as a histidine kinase receptor and a response regulator respectively.
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AgrBDCA is a quorum sensing system found to regulate virulence factors in <i>Staphylococcus aureus</i>. In this system, agrC and agrA function as a histidine kinase receptor and a response regulator respectively. This subproject aimed to introduce agrC and agrA in functioning form into <i>E. coli</i>.
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Additionally, this subgroup focused on prototyping various combinatorial promoters--those which accept multiple inputs in order to determine an output state. This part of the subproject stemmed from the original overall project idea, which was to characterize a negative feedback loop with extracellular components and required a combinatorial promoter to control production of the ligand exported out of the cell. Since work had already begun on that project, it was continued, even after the shift in project design and focus, in hopes that it will later be incorporated again in the circuit.
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<h4>FsrABC Signal Reception</h4>
<h4>FsrABC Signal Reception</h4>
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FsrABC is another quorum sensing system found to regulate virulence factors in Entereococcus Faecialis (sp?). In this system, FsrC and FsrA function as histidine kinase receptor and response regulator respectively. FsrB is a membrane protein that is processed to form a cyclic 11-amino-acid polypeptide <!-- add a tag here that brings you down to Ligand export systems section -->(see Ligand Export Systems).
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FsrABC is another quorum sensing system homologous to the agrBCDA system of <i>S. aureus</i> and is also believed to regulate virulence factors involved in biofilm formation in <i>Entereococcus faecalis</i>. In this system, FsrC and FsrA function as histidine kinase receptor and response regulator respectively. FsrB is a membrane protein that is processed to form a cyclic 11-amino-acid polypeptide <!-- add a tag here that brings you down to Ligand export systems section -->(see Ligand Export Systems).
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lamBCDA is a third quorum sensing system native to <i>Lactobacillus Plantarum</i> that also shares homology with the agrBCDA QS system in <i>S. aureus</i>. Normally, it regulates
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<center><img src="https://static.igem.org/mediawiki/2014/5/5f/Fsr_system.png" alt="Fsr System"></center>
<center><img src="https://static.igem.org/mediawiki/2014/5/5f/Fsr_system.png" alt="Fsr System"></center>

Revision as of 18:52, 10 July 2014


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Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Project Overview

Our project was split into 3 subprojects: AgrBDCA signal reception & Combinatorial Promoters, FsrABC/lamBCDA signal reception, and Ligand export systems.

AgrBDCA Signal Reception & Combinatorial Promoters

AgrBDCA is a quorum sensing system found to regulate virulence factors in Staphylococcus aureus. In this system, agrC and agrA function as a histidine kinase receptor and a response regulator respectively. This subproject aimed to introduce agrC and agrA in functioning form into E. coli.

Additionally, this subgroup focused on prototyping various combinatorial promoters--those which accept multiple inputs in order to determine an output state. This part of the subproject stemmed from the original overall project idea, which was to characterize a negative feedback loop with extracellular components and required a combinatorial promoter to control production of the ligand exported out of the cell. Since work had already begun on that project, it was continued, even after the shift in project design and focus, in hopes that it will later be incorporated again in the circuit.

FsrABC Signal Reception

FsrABC is another quorum sensing system homologous to the agrBCDA system of S. aureus and is also believed to regulate virulence factors involved in biofilm formation in Entereococcus faecalis. In this system, FsrC and FsrA function as histidine kinase receptor and response regulator respectively. FsrB is a membrane protein that is processed to form a cyclic 11-amino-acid polypeptide (see Ligand Export Systems).

lamBCDA is a third quorum sensing system native to Lactobacillus Plantarum that also shares homology with the agrBCDA QS system in S. aureus. Normally, it regulates

Fsr System

Ligand Export Systems

With the AgrBDCA system, it is believed that agrB is a membrane protein that exports and modifies a short polypeptide sequence into a polypeptide ligand to be bound by agrC, the histidine kinase receptor.

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