Team:SCU-China/Digestion

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Revision as of 22:29, 16 October 2014

Digestion Protocol

According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20μl).

DNA

250ng

Enzyme 1

1μl

Enzyme 2

1μl

10 X Buffer

2μl

H2O

Up to 20μl

Sichuan university

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