Team:Bielefeld-CeBiTec/Results/DataPage

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<p>
<p>
This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project">project part</a> of the Wiki.</p>  
This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project">project part</a> of the Wiki.</p>  
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<font size="2" style="text-align:left;"><b>Figure 1</b>: Project description</font>
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<font size="2" style="text-align:left;"><b>Figure 1</b>: Project description</font>
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<h6> Data for rMFC</h6>
<h6> Data for rMFC</h6>
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<h6> Data for CO<sub>2</sub> Fixation</h6>
<h6> Data for CO<sub>2</sub> Fixation</h6>
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<h6> Data for Isobutanol</h6>
<h6> Data for Isobutanol</h6>
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<table style="background-color:transparent">

Revision as of 22:00, 16 October 2014


Data Page

This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our project part of the Wiki.

Figure 1: Project description
Data for rMFC
BioBrickDescription
BBa_K1465101Cytochrome c from Geobacter sulfurreducens for electron transfer with mediators for reduction equivalent generation
BBa_K1465102Fumerate reductase (frd) from E. coli under the control of the T7 promoter
BBa_K1465103Fumerate reductase (FumABCD) from Actinobacillus succinogenes
BBa_K1465104Fumarate reductase FumBCD from Actinobacillus succinogenes
BBa_K1465105Fumarate reductase (Fum) under control of the T7 promoter
BBa_K1465107Primer sequence for genome editing of porine fwd
BBa_K1465108Primer sequence for genome editing of porine rev
BBa_K1465109Primer sequence for genome editing of dcuB fwd
BBa_K1465110Primer sequence for genome editing of dcuB rev
Data for CO2 Fixation
BioBrickDescription
BBa_K1465201Phosphoribulokinase prkA from Synechoccus elongatus, codon optimized for E. coli.
BBa_K1465202Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus
BBa_K1465203Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechoccus elangatus.
BBa_K1465204Shell-associated protein (csoS2) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465205Carbonic anhydrase (csoS3) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465206shell proteins (csoS4) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465207pore-forming protein (csoS1D) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465208can + shell proteins (cloning intermediate) from Halothiobacillus neapolitanus
BBa_K1465209Carbonic anhydrase + shell proteins + csoS1D from Halothiobacillus neapolitanus
BBa_K1465212Phosphoribulokinase prkA from Synechoccus elongatus under the control of the ptac promotor
BBa_K1465213RubisCO from Halothiobacillus neapolitanus under control of the ptac promoter
BBa_K1465214Phosphoribulokinase of Halothiobacillus neapolitanus under control of ptac
BBa_K1465222shell-protein fused with GFP
BBa_K1465223Empty Carboxysome with GFP
BBa_K1465225Knock-down with sRNA of phosphofructo kinase. (sRNA:pfkA)
BBa_K1465226Knock-down with sRNA of phosphofructo kinase under control of ptac promotor (ptac_sRNA:pfkA)
BBa_K1465227Knock-down with sRNA of phosphofructo kinase under control of T7 promotor. (t7_sRNA:pfkA)
BBa_K1465228Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus
BBa_K1465229Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus under the control of the ptac promotor
BBa_K1465230Purification vector (T7-RBS Intein-tag)
Data for Isobutanol
BioBrickDescription
BBa_K1465301Alochol dehydrogenase from Lactococcus lactis
BBa_K1465302Isobutanol producing pathway (alsS_ilvC_ilvD_kivD)
BBa_K1465303Isobutanol producing pathway combined with alcohol dehydrogenase (alsS_ilvC_ilvD_kivD_adhA)
BBa_K1465304Alcohol dehydrogenase under control of T7 promotor
BBa_K1465305Alcohol dehydrogenase under control of ptac promotor
BBa_K1465306Isobutanol producing pathway under control of ptac promotor
BBa_K1465307Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promotor
Data for Biosafety
BioBrickDescription
BBa_K1465401Alanin racemase (alr) knock-out strain fused with RFP as visual control for transformation in E.coli
BBa_K1465403Primer for alanin racemase (alr) fwd
BBa_K1465404Primer for alanin racemase (alr) rev
BBa_K1465405Primer for dadX fwd
BBa_K1465406Primer for dadX rev

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