Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Assay to detect ''P. aeruginosa'' using ''Cellock Holmes''.|subtitle=This flow sheet shows the procedure to sample and detect ''P. aeruginosa''. Briefly a sampling chip is put onto the potentially contaminated surface, added onto one of our sensor chips and inserted into ''WatsOn''.|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Assay to detect ''P. aeruginosa'' using ''Cellock Holmes''.|subtitle=This flow sheet shows the procedure to sample and detect ''P. aeruginosa''. Briefly a sampling chip is put onto the potentially contaminated surface, added onto one of our sensor chips and inserted into ''WatsOn''.|width=1000px}} | ||
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Using ''Cellock Holmes'', we developed a simple assay to detect ''P. aeruginosa''. | Using ''Cellock Holmes'', we developed a simple assay to detect ''P. aeruginosa''. | ||
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* Second, the sampling chip is removed from the surface and put onto one of our sensor chips. (Theorectically, the sensor chips could be directly used for sampling, however, this was avoided in our project to '''match [https://2014.igem.org/Team:Aachen/Safety biosafety regulations]''' and to prevent the spread of GMOs into the environment.) | * Second, the sampling chip is removed from the surface and put onto one of our sensor chips. (Theorectically, the sensor chips could be directly used for sampling, however, this was avoided in our project to '''match [https://2014.igem.org/Team:Aachen/Safety biosafety regulations]''' and to prevent the spread of GMOs into the environment.) | ||
* Third, the two layered chip-stack is put into a petri dish which is inserted into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] for evalutation. | * Third, the two layered chip-stack is put into a petri dish which is inserted into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] for evalutation. | ||
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside ''WatsOn''.|subtitle=Chips are incubated at 37 °C to stimulate cell growth and then illuminated with blue light to excite fluorescence. A picture is taken and analyzed for fluorescence signals using the software ''Measurarty''.|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside ''WatsOn''.|subtitle=Chips are incubated at 37 °C to stimulate cell growth and then illuminated with blue light to excite fluorescence. A picture is taken and analyzed for fluorescence signals using the software ''Measurarty''.|width=1000px}} |
Revision as of 20:52, 16 October 2014
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