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Team:Caltech/week4
From 2014.igem.org
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| - | <ul> | + | <ul><li>PCR extracted backbone from pKS001 plasmid with overhangs for Biobrick prefixes & suffixes</li> |
| + | <li>PCR purification of PCR product and then 1 hour incubation at 37°C with DPN1 enzyme to remove any remaining sticky ends</li> | ||
| + | <li>PCR purification of DPN1-digested product</li> | ||
| + | <li>Ran a gel that confirmed presence of ~3000bp-long DNA fragment in both DPN1-digested and undigested PCR products</li> | ||
| + | <li>Gibson Assembly of the PCR fragments with the geneblocks ordered 2 weeks ago to form exportDomain-comX-mNeonGreen constructs in the vector backbone</li> | ||
| + | <li>Transformation of assemblies into JM109. Plated on ampicillin plates and incubated at 37°C overnight</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
Revision as of 01:13, 8 July 2014
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