Team:SCU-China

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
{{CSS/Main}}
{{CSS/Main}}
-
 
-
 
<html>
<html>
 +
<style>
 +
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
 +
</style>
 +
<link rel="stylesheet" href="https://2014.igem.org/Team:SCU-China/bootstrap.min.css ?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css"/>
 +
<link rel="stylesheet" href="https://2014.igem.org/Team:SCU-China/bootstrap-theme.min.css ?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css"/>
-
<!--main content -->
 
-
<table width="70%" align="center">
 
 +
<script src="https://2014.igem.org/Team:SCU-China/jquery.min.js ?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:SCU-China/bootstrap.min.js ?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
 +
<nav class="navbar navbar-default navbar-fixed-top" role="navigation" style="margin-top: 20px;">
 +
      <div class="container">
 +
        <div class="navbar-header">
 +
          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar">
 +
            <span class="sr-only">Toggle navigation</span>
 +
            <span class="icon-bar"></span>
 +
            <span class="icon-bar"></span>
 +
            <span class="icon-bar"></span>
 +
          </button>
 +
          <a class="navbar-brand" href="#">SCU-Igem</a>
 +
        </div>
 +
        <div id="navbar" class="collapse navbar-collapse">
 +
          <ul class="nav navbar-nav">
 +
            <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project <span class="caret"></span></a>
 +
              <ul class="dropdown-menu" role="menu">
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Background">Background</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Overview">Overview</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Description">Description</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Result">Result</a></li>
 +
               
 +
              </ul>
 +
            </li>
 +
            <li><a href="https://2014.igem.org/Team:SCU-China/Modeling">Modeling</a></li>
 +
            <li><a href="https://2014.igem.org/Team:SCU-China/HumanPractice">Human Practice</a></li>
 +
            <li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a></li>
 +
            <li><a href="https://2014.igem.org/Team:SCU-China/Team">Team</a></li>
 +
            <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Notbook<span class="caret"></span></a>
 +
              <ul class="dropdown-menu" role="menu">
 +
                <li class="dropdown-header">Notebook</li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Transmitter">Notebook of Transmitter</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Effector">Notebook of Effector</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Sensor">Notebook of Sensor</a></li>
 +
                <li class="divider"></li>
 +
                <li class="dropdown-header">Method</li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Prep">Bacterial Genomic DNA Prep</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Digestionk">Digestionk</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/GelExtraction">Gel Extraction </a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Linkage">Linkage</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/PCR">PCR</a></li>
 +
                <li><a href="https://2014.igem.org/Team:SCU-China/Plasmid">Plasmid Mini Prep</a></li>
 +
              </ul>
 +
            </li>
 +
          </ul>
 +
        <li style="float:right"><a href="https://2014.igem.org" style="padding-left: 10px; padding-right: 10px;"><img src="https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png"></a></li>
 +
        </div><!--/.nav-collapse -->
 +
   
 +
   
 +
    </nav>
 +
    <div class="jumbotron" style="margin-top: 20px;"> <div class="container">
 +
  <h1>Imperial Edict</h1>
 +
  <p>Simulating Signal Analysis & Transfer Using E.coli</p>
 +
  <p><img src="https://static.igem.org/mediawiki/2014/5/52/Scuhome.png"></p>
 +
</div></div>
 +
    <div class="container" style="border-bottom-color: #676767;
 +
border-bottom-width: thick;">
 +
    <div class="col-lg-12">
 +
       
 +
          <p>Our project is focusing on using the simple prokaryotes to imitate the nerve system which exists in the advanced animals widely. As we all known, the nerve system of advanced animals like human consists of 3 major parts: the receptive neurons, the central neurons, and the effector cells. </p><p>Correspondingly, we separate bacteria into 3 different communities to execute different functions and we name these 3 communities as Receptor, Transmitter, and Effector.</p><p> In details, we replace the neurotransmitter by AHL family and in the meanwhile, we construct the multiple promoter to simulate the procedure of signal receiving and processing.</p><p> In addition, for the sake of achieving the signal transmitting and analyzing, we isolate a new cell quorum sensing system—the PPYs signal pathway from photorhabdus luminescence. The mechanism of this PPYs signal pathway is similar to the AHLs pathway. The PPYs protein could catalyze the generation of ppys, a kind of small molecule which is similar to the AHL and HSL.</p><p> And then, ppys could combine with PluR protein to activate the Pppy promoter.</p>
 +
  </div></div>
 +
 +
<div class="container">
 +
<div class="panel panel-default">
 +
  <div class="panel-body">
 +
 
 +
<div class="col-lg-6">
 +
 +
<h1>comic</h1>
 +
<p>Some day, the emperor(cell 1) wants to know the people's life(stimulation), so he told the minister(cell 2), (1st signal). </p><p>Then, the minister went out and told the different representative of normal people(three kinds of cell 3) about this(2nd signal and signal distribution).</p><p> Later, the representative of civilians showed the emperor with their specialities.(effect: express different fluorescent protein )
 +
</p></div>
-
<!--welcome box -->
+
<div class="col-lg-6"><p><img src="https://static.igem.org/mediawiki/2014/a/a5/Scucomic.png"></p></div></div></div>
-
<tr>
+
</div>
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
<div class="jumbotron"> <div class="container">
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
 
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
  <p>Sichuan university</p>
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
-
<br>
+
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCU-China&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
<!-- end welcome box -->
+
-
<tr>
+
-
 
+
-
<!--navigation menu -->
+
-
<td align="center" colspan="3">
+
-
 
+
-
<table  width="100%">
+
-
<tr heigth="15px"></tr>
+
-
<tr heigth="75px">
+
-
 
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:SCU-China"style="color:#000000">Home </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:SCU-China/Team"style="color:#000000"> Team </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=SCU-China"style="color:#000000"> Official Team Profile </a></td>
+
-
 
+
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:SCU-China/Project"style="color:#000000"> Project</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:SCU-China/Parts"style="color:#000000"> Parts</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
-
<a href="https://2014.igem.org/Team:SCU-China/Modeling"style="color:#000000"> Modeling</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:SCU-China/Notebook"style="color:#000000"> Notebook</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:SCU-China/Safety"style=" color:#000000"> Safety </a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:SCU-China/Attributions"style="color:#000000"> Attributions </a></td>
+
-
 
+
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
 
+
-
 
+
-
</tr>
+
   
   
-
 
+
</div></div>
-
 
+
-
 
+
-
 
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<tr><td colspan="3"> <h3> Project Description </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
 
+
-
<p> Our project is focusing on using the simple prokaryotes to imitate the nerve system which exists in the advanced animals widely. As we all known, the nerve system of advanced animals like human consists of 3 major parts: the receptive neurons, the central neurons, and the effector cells. Correspondingly, we separate bacteria into 3 different communities to execute different functions and we name these 3 communities as Receptor, Transmitter, and Effector. In details, we replace the neurotransmitter by AHL family and in the meanwhile, we construct the multiple promoter to simulate the procedure of signal receiving and processing. </p>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>  In addition, for the sake of achieving the signal transmitting and analyzing, we isolate a new cell quorum sensing system—the PPYs signal pathway from photorhabdus luminescence. The mechanism of this PPYs signal pathway is similar to the AHLs pathway. The PPYs protein could catalyze the generation of ppys, a kind of small molecule which is similar to the AHL and HSL. And then, ppys could combine with PluR protein to activate the Pppy promoter.</p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
<h1 align="center" >Imperial Edict:Simulating Signal Analysis & Transfer Using E.coli</h1>
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
<!--requirements section -->
+
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
 
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
-
 
+
-
<!-- Links to other team pages -->
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=SCU-China">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a> </li>
+
-
 
+
-
</ul>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>There are a few wiki requirements teams must follow:</p>
+
-
<ul>
+
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
+
-
<li>All pages must be created under the team’s name space.</li>
+
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
+
-
<li>Do not use flash in wiki code. </li>
+
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
+
-
</ul>
+
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
 
+
-
 
+
-
<!--tips  -->
+
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
 
+
-
<tr>
+
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
 
+
-
<ul>
+
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
 
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
-
 
+
-
<td> </td>
+
-
<td width="45%">
+
-
 
+
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
-
 
+
-
<ul>
+
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
-
<li>Have lots of fun! </li>
+
-
</ul>
+
-
</br>
+
-
</td>
+
-
</tr>
+
-
</table>
+

Revision as of 16:34, 16 October 2014

Imperial Edict

Simulating Signal Analysis & Transfer Using E.coli

Our project is focusing on using the simple prokaryotes to imitate the nerve system which exists in the advanced animals widely. As we all known, the nerve system of advanced animals like human consists of 3 major parts: the receptive neurons, the central neurons, and the effector cells.

Correspondingly, we separate bacteria into 3 different communities to execute different functions and we name these 3 communities as Receptor, Transmitter, and Effector.

In details, we replace the neurotransmitter by AHL family and in the meanwhile, we construct the multiple promoter to simulate the procedure of signal receiving and processing.

In addition, for the sake of achieving the signal transmitting and analyzing, we isolate a new cell quorum sensing system—the PPYs signal pathway from photorhabdus luminescence. The mechanism of this PPYs signal pathway is similar to the AHLs pathway. The PPYs protein could catalyze the generation of ppys, a kind of small molecule which is similar to the AHL and HSL.

And then, ppys could combine with PluR protein to activate the Pppy promoter.

comic

Some day, the emperor(cell 1) wants to know the people's life(stimulation), so he told the minister(cell 2), (1st signal).

Then, the minister went out and told the different representative of normal people(three kinds of cell 3) about this(2nd signal and signal distribution).

Later, the representative of civilians showed the emperor with their specialities.(effect: express different fluorescent protein )

Sichuan university