Team:TU Darmstadt/Notebook/Methods/DNA quantification with NanoDrop

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Revision as of 16:08, 16 October 2014

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DNA quantification with NanoDrop

Equipment:

- Micropipettes with sterile tips

- Nanodrop

Chemicals & consumables: 

- DNA probe

- nuclease free H2O

Procedure:

1. Start the programme for the NanoDrop and click on "Nucleic Acids"

2. Pipette 1 µL of a water sample onto the measuring surface and click “OK” for initialising

3. Load your blank and click "Blank"

4. Load your DNA samples and click "Measure"

The system calculate automatically the 260/280 value and the 260/230 value.