"
Team:HokkaidoU Japan/Notebook/Pre experiment/mRFP-expression-construct
From 2014.igem.org
(Difference between revisions)
| Line 302: | Line 302: | ||
</div> | </div> | ||
</div> | </div> | ||
| - | <div id="footer-wrapper" style="background-color:# | + | <!--begin footer--> |
| - | <div id="footer- | + | |
| - | + | <div id="footer-wrapper" style="background-color: #d92424;"> | |
| - | + | <div id="footer-content"> | |
| - | + | <div id="footer-logo"> | |
| - | + | <a href="http://igemhokkaidou.com/"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a> | |
| - | + | ||
</div> | </div> | ||
| + | <div id="footer-twitter"> | ||
| + | <a href="http://twitter.com/igem_hokkaidou"><img style="height:150px; position:relative; bottom:0;" src="https://static.igem.org/mediawiki/2014/3/3b/HokkaidoU_Footer_Twitter.png"></a> | ||
| + | </div> | ||
| - | < | + | </div> |
| + | </div> | ||
</div><!--background--> | </div><!--background--> | ||
| + | <link href='http://fonts.googleapis.com/css?family=Special+Elite' rel='stylesheet' type='text/css'> | ||
</html> | </html> | ||
{{Team:HokkaidoU_Japan/JS}} | {{Team:HokkaidoU_Japan/JS}} | ||
Revision as of 13:39, 16 October 2014
hogehoge
mRFP Expression Plasmid Construction
-
Start
-
Get genes
pSB6A1 -
Transformation
pSB6A1 1µL DNA to JM109 -
Liquid Culture
pSB6A1 -
Mini-prep
pSB6A1 -
PCR & PCR purification
R0040-B0034-E1010-B0015 from our stock 100up 200dn -
Digestion
Cut R0040-B0034-E1010-B0015 with EcoRI, PstI (using 10×H buffer) Cut pSB6A1 with EcoRI, PstI (using 10×H buffer) -
Gel Extraction & Ethanol precipetation
R0040-B0034-E1010-B0015 pSB6A1 -
Dephosphorylation
R0040-B0034-E1010-B0015 -
Ligation
Ligate R0040-B0034-E1010-B0015 with pSB6A1 -
Transformation
R0040-B0034-E1010-B0015 on pSB6A1 5µL DNA to DH5α Turbo -
Liquid Culture
R0040-B0034-E1010-B0015 on pSB6A1 -
Mini-prep
R0040-B0034-E1010-B0015 on pSB6A1 -
Complete!
