Team:CityU HK/project/result

From 2014.igem.org

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   <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li>
   <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li>
   <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li>
   <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li>
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   <li class='last'><a href='#'><span>Desaturase Module</span></a></li>
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   <li class='last'><a href='https://2014.igem.org/Team:CityU_HK/project/result_desaturase'><span>Desaturase Module</span></a></li>
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Revision as of 13:31, 16 October 2014

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Result Summary

Experimental Achievements from 15 June – 3 October 2014 :

Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).

Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.

Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).

Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.

Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.

Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).

Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.

Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.



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