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Team:NRP-UEA-Norwich/Notebook Protocols
From 2014.igem.org
(Difference between revisions)
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<div id="accordion"> | <div id="accordion"> | ||
| - | <h3 class="accordion-toggle"> | + | <h3 class="accordion-toggle"> GoldenGate Digestion-Ligation reaction (Level 1) </h3> |
<div class="accordion-content"> | <div class="accordion-content"> | ||
PROTOCOL HERE | PROTOCOL HERE | ||
</div> | </div> | ||
| - | <h3 class="accordion-toggle"> | + | <h3 class="accordion-toggle"> GoldenGate Digestion-Ligation reaction (Level 2)</h3> |
<div class="accordion-content"> | <div class="accordion-content"> | ||
PROTOCOL HERE | PROTOCOL HERE | ||
</div> | </div> | ||
| - | + | <h3 class="accordion-toggle">Making LB Agar</h3> | |
<div class="accordion-content"> | <div class="accordion-content"> | ||
PROTOCOL HERE | PROTOCOL HERE | ||
</div> | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">Making LB broth</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle"><i>E. coli</i> Calcium Chloride heat shock Transformation</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | |||
<h3 class="accordion-toggle"><i>E. coli</i> Electroporation Transformation</h3> | <h3 class="accordion-toggle"><i>E. coli</i> Electroporation Transformation</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">Making elctrocompetent <i>Agrobacterium tumefaciens</i> </h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <h3 class="accordion-toggle"><i>Agrobacterium tumefaciens</i> Electroporation Transformation</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <h3 class="accordion-toggle">Blue- White Selection</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">Colony PCR</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">DNA mini-prep</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">Preparation for sequencing</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <h3 class="accordion-toggle"><i>Agrobacterium tumefaciens</i> Infiltration</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle">Infiltration Analysis</h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle"> Making GoldenGate compatible PSB1C3 'Flipper' </h3> | ||
| + | <div class="accordion-content"> | ||
| + | PROTOCOL HERE | ||
| + | </div> | ||
| + | |||
| + | <h3 class="accordion-toggle"> Antibiotic Selection </h3> | ||
<div class="accordion-content"> | <div class="accordion-content"> | ||
PROTOCOL HERE | PROTOCOL HERE | ||
Revision as of 12:14, 16 October 2014
Lab Protocols
GoldenGate Digestion-Ligation reaction (Level 1)
PROTOCOL HERE
GoldenGate Digestion-Ligation reaction (Level 2)
PROTOCOL HERE
Making LB Agar
PROTOCOL HERE
Making LB broth
PROTOCOL HERE
E. coli Calcium Chloride heat shock Transformation
PROTOCOL HERE
E. coli Electroporation Transformation
PROTOCOL HERE
Making elctrocompetent Agrobacterium tumefaciens
PROTOCOL HERE
Agrobacterium tumefaciens Electroporation Transformation
PROTOCOL HERE
Blue- White Selection
PROTOCOL HERE
Colony PCR
PROTOCOL HERE
DNA mini-prep
PROTOCOL HERE
Preparation for sequencing
PROTOCOL HERE
Agrobacterium tumefaciens Infiltration
PROTOCOL HERE
Infiltration Analysis
PROTOCOL HERE
Making GoldenGate compatible PSB1C3 'Flipper'
PROTOCOL HERE
Antibiotic Selection
PROTOCOL HERE
