Team:Aachen/Parts

From 2014.igem.org

(Difference between revisions)
(→K1319000)
Line 4: Line 4:
==[http://parts.igem.org/Part:BBa_K1319000 K1319000]==
==[http://parts.igem.org/Part:BBa_K1319000 K1319000]==
 +
<span class="anchor" id="partsK1319000"></span>
===RFC &#91;25&#93; Version of E0020===
===RFC &#91;25&#93; Version of E0020===
Line 14: Line 15:
==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
 +
<span class="anchor" id="partsK1319001"></span>
===RFC [25] - compatible dark quencher based on K1319000 (E0030)===
===RFC [25] - compatible dark quencher based on K1319000 (E0030)===
Line 26: Line 28:
==[http://parts.igem.org/Part:BBa_K1319002 K1319002]==
==[http://parts.igem.org/Part:BBa_K1319002 K1319002]==
 +
<span class="anchor" id="partsK1319002"></span>
===RFC [25] - compatible dark quencher based on K1319000 (E0030)===
===RFC [25] - compatible dark quencher based on K1319000 (E0030)===
Line 39: Line 42:
==[http://parts.igem.org/Part:BBa_K1319003 K1319003]==
==[http://parts.igem.org/Part:BBa_K1319003 K1319003]==
 +
<span class="anchor" id="partsK1319003"></span>
===human galectin-3, codon optimized for ''E. coli''===
===human galectin-3, codon optimized for ''E. coli''===
Line 47: Line 51:
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==
 +
<span class="anchor" id="partsK1319004"></span>
===TEV protease with anti-self cleavage mutation S219V, codon optimized for ''E. coli''===
===TEV protease with anti-self cleavage mutation S219V, codon optimized for ''E. coli''===
Line 61: Line 66:
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==
 +
<span class="anchor" id="partsK1319008"></span>
=== IPTG-induced and T7-driven expression of TEV protease ===
=== IPTG-induced and T7-driven expression of TEV protease ===
Line 69: Line 75:
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==
 +
<span class="anchor" id="partsK1319010"></span>
=== Constitutive expression of K1319000 ===
=== Constitutive expression of K1319000 ===
Line 77: Line 84:
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==
 +
<span class="anchor" id="partsK1319011"></span>
=== Constitutive expression of K1319001 ===
=== Constitutive expression of K1319001 ===
Line 85: Line 93:
== [http://parts.igem.org/Part:BBa_K1319012 K1319012] ==
== [http://parts.igem.org/Part:BBa_K1319012 K1319012] ==
 +
<span class="anchor" id="partsK1319012"></span>
=== Constitutive expression of K1319002 ===
=== Constitutive expression of K1319002 ===
Line 93: Line 102:
== [http://parts.igem.org/Part:BBa_K1319013 K1319013] ==
== [http://parts.igem.org/Part:BBa_K1319013 K1319013] ==
 +
<span class="anchor" id="partsK1319013"></span>
=== Constitutive expression of GFP-REACh1 fusion protein ===
=== Constitutive expression of GFP-REACh1 fusion protein ===
Line 101: Line 111:
== [http://parts.igem.org/Part:BBa_K1319014 K1319014] ==
== [http://parts.igem.org/Part:BBa_K1319014 K1319014] ==
 +
<span class="anchor" id="partsK1319014"></span>
===Constitutive expression of GFP-REACh2 fusion protein===
===Constitutive expression of GFP-REACh2 fusion protein===
Line 109: Line 120:
== [http://parts.igem.org/Part:BBa_K1319015 K1319015] ==
== [http://parts.igem.org/Part:BBa_K1319015 K1319015] ==
 +
<span class="anchor" id="partsK1319015"></span>
===Constitutive expression of GFP-EYFP fusion protein===
===Constitutive expression of GFP-EYFP fusion protein===
Line 117: Line 129:
== [http://parts.igem.org/Part:BBa_K1319016 K1319016] ==
== [http://parts.igem.org/Part:BBa_K1319016 K1319016] ==
 +
<span class="anchor" id="partsK1319016"></span>
===TEV protease cleavage site===
===TEV protease cleavage site===
Line 127: Line 140:
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==
 +
<span class="anchor" id="partsK1319017"></span>
=== LasI induced iLOV ===
=== LasI induced iLOV ===
Line 135: Line 149:
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==
 +
<span class="anchor" id="partsK1319020"></span>
===Translational unit of mRFP-galectin3-His===
===Translational unit of mRFP-galectin3-His===
Line 143: Line 158:
==[http://parts.igem.org/Part:BBa_K1319042 K1319042]==
==[http://parts.igem.org/Part:BBa_K1319042 K1319042]==
 +
<span class="anchor" id="partsK1319042"></span>
===IPTG inducible iLOV===
===IPTG inducible iLOV===

Revision as of 08:17, 16 October 2014

iGEM Team Aachen BioBricks

[http://parts.igem.org/Part:BBa_K1319000 K1319000]

RFC [25] Version of E0020

This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.

The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from A. victoria GFP. The excitation is 512 nm and the emission is 534 nm.

[http://parts.igem.org/Part:BBa_K1319001 K1319001]

RFC [25] - compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Two mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W

[http://parts.igem.org/Part:BBa_K1319002 K1319002]

RFC [25] - compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W
  • H148R

[http://parts.igem.org/Part:BBa_K1319003 K1319003]

human galectin-3, codon optimized for E. coli

The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.

[http://parts.igem.org/Part:BBa_K1319004 K1319004]

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

This part is a TEV protease in RFC25 that was codon-optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

The TEV Protease (also known as Tobaco Edge Virus nuclear inclusion a endopeptidase) is a highly sequence specific cysteine protease from the Tobacco Edge Virus (TEV). The protease is highly sequence specific. The consensus sequence for the cut is ENLYFQ\S with \ denoting the cleaved peptide bond. This sequence can be found in the part [http://parts.igem.org/Part:BBa_K1319016 K1319016].

ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.

The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like [http://parts.igem.org/Part:BBa_K1319007 His-Tags]. The high specifity makes the protease relatively non-toxic in vitro and in vivo. The molecular weight of the TEV protease is 27 kDa.

[http://parts.igem.org/Part:BBa_K1319008 K1319008]

IPTG-induced and T7-driven expression of TEV protease

This protein generator produces [http://parts.igem.org/Part:BBa_K1319004 TEV protease] when induced with IPTG in a DE3 strain or if combined with a T7 RNA-Polymerase generator.

[http://parts.igem.org/Part:BBa_K1319010 K1319010]

Constitutive expression of K1319000

This part expresses K1319000 behind a J23101 constitutive promotor.

[http://parts.igem.org/Part:BBa_K1319011 K1319011]

Constitutive expression of K1319001

This part expresses K1319001 behind a J23101 constitutive promotor.

[http://parts.igem.org/Part:BBa_K1319012 K1319012]

Constitutive expression of K1319002

This part expresses K1319002 behind a J23101 constitutive promotor.

[http://parts.igem.org/Part:BBa_K1319013 K1319013]

Constitutive expression of GFP-REACh1 fusion protein

This part expresses a E0040.K1319001 fusion protein (GFP-REACh1) behind a J23101 promotor.

[http://parts.igem.org/Part:BBa_K1319014 K1319014]

Constitutive expression of GFP-REACh2 fusion protein

This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promotor.

[http://parts.igem.org/Part:BBa_K1319015 K1319015]

Constitutive expression of GFP-EYFP fusion protein

This part expresses a E0040.K1319000 fusion protein (GFP-EYFP) behind a J23101 promotor.

[http://parts.igem.org/Part:BBa_K1319016 K1319016]

TEV protease cleavage site

This sequence codes for a [http://parts.igem.org/Part:BBa_K1319004 TEV protease] cleavage site.

ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.

[http://parts.igem.org/Part:BBa_K1319017 K1319017]

LasI induced iLOV

This device produces iLOV (K660004) in response to a quorum sensing input.

[http://parts.igem.org/Part:BBa_K1319020 K1319020]

Translational unit of mRFP-galectin3-His

This part is a translational unit of a mRFP-galectin-3-His (B0032.E1010.K1319003.K1319016.B0015).

[http://parts.igem.org/Part:BBa_K1319042 K1319042]

IPTG inducible iLOV

This part can be used for IPTG-induced expression of K660004 (iLOV).



  1. Part Name
  2. Part type
  3. Creator
  4. Sequence
  5. Short Description (60 characters on what the DNA does)
  6. Long Description (Longer description of what the DNA does)
  7. Design considerations


<groupparts>iGEM14 Aachen</groupparts>

Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Parts"