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Team:Bielefeld-CeBiTec/Project/Isobutanol/GeneticalApproach
From 2014.igem.org
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<img src="https://static.igem.org/mediawiki/2014/8/8a/Bielefeld_CeBiTec_2014-10-16_Isobutanol_pathway.png" width="400px" align="center"> | <img src="https://static.igem.org/mediawiki/2014/8/8a/Bielefeld_CeBiTec_2014-10-16_Isobutanol_pathway.png" width="400px" align="center"> | ||
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| + | <font size="2" style="text-align:left;"><b>Figure 1</b>: Schematic illustration of the isobutanol pathway</font> | ||
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Revision as of 08:05, 16 October 2014
Isobutanol
Genetical Approach
There are different genetical approaches to produce isobutanol with various organisms like cyanobacteria, B. subtilis , E. coli and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012 in E. coli. The iGEM Team Formosa identified the four proteins alsS, ilvC, ilvD and kivD as required for the production of isobutanol in E. coli. We want to reproduce their idea without the temperature shift and with all genes on one plasmid. Additionally we want to combine this plasmid with the adhA from L. lactis.
Figure 1: Schematic illustration of the isobutanol pathway
