Team:UESTC-China/result
From 2014.igem.org
(Difference between revisions)
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<h1 class="SectionTitles" style="width:900px; ">Expression of four key enzymes in tobacco</h1><br/> | <h1 class="SectionTitles" style="width:900px; ">Expression of four key enzymes in tobacco</h1><br/> | ||
- | <p style="color:#1b1b1b;">We extracted DNA from tobacco plantlets. And then we used specific primers to amplify the target gene to verify the kan-resistant seedlings (Fig. | + | <p style="color:#1b1b1b;">We extracted DNA from tobacco plantlets. And then we used specific primers to amplify the target gene to verify the kan-resistant seedlings (Fig.6). Next we extracted RNA from tobacco leaves which are PCR positive. We used RT-PCR to detect whether target gene was expressed (Fig.7).</p> |
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<div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/5/58/10.png"> | <div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/5/58/10.png"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"><strong>Fig. | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"><strong>Fig.6</strong> |
PCR identification of kan-resistant tobacco seedlings (piGEM010 transgenic line). | PCR identification of kan-resistant tobacco seedlings (piGEM010 transgenic line). | ||
M: DNA marker; | M: DNA marker; | ||
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<div align="center"><img style="width:45% ;" src="https://static.igem.org/mediawiki/2014/a/af/RNA%2BACTINJPG.JPG"> | <div align="center"><img style="width:45% ;" src="https://static.igem.org/mediawiki/2014/a/af/RNA%2BACTINJPG.JPG"> | ||
<p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> | ||
- | <strong>Fig. | + | <strong>Fig.7</strong> RT-PCR verification of positive transgenic tobacco seedlings.<br/> |
M: DNA marker; 1-8: 6 individual lines; 9: plasmid | M: DNA marker; 1-8: 6 individual lines; 9: plasmid | ||
</p> | </p> |
Revision as of 03:48, 16 October 2014