Team:Cornell/project/drylab/testing
From 2014.igem.org
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<h1>Luria Broth Testing</h1> | <h1>Luria Broth Testing</h1> | ||
- | + | One factor in the longevity of living cells in our fiber reactor system is the retention time of growth media in the outer chamber. To test this retention time, a test was conducted to see how long it took for LB to flow out of the fiber reactor. Solutions of LB and tonic water of varying concentrations were run through the fiber reactor, and the amount of solution that came out of the filter, as well as the concentration of solution, were measured. This was done by using tonic water, which fluoresces under UV light because it contains quinine. Overall, it was determined that LB does not remain in the fiber reactor for a long enough time to support growth. To support long-term growth we would have to incorporate a separate media cycling system into the reactor. However, this reactor system is designed to work with dormant cells that should not require media for growth, so this is not a huge concern for our project. | |
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Revision as of 03:17, 16 October 2014