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Team:TU Darmstadt/Notebook/Methods/hydrochloric acid extraction
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Revision as of 01:28, 16 October 2014
37% hydrochloric acid extraction
Materials:
- 37% hydrochloric acid
- Centrifuge
- Tubes
Procedure:
Spin down the bacterial solution at 4500g for 10 minutes.
Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.
Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.
The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.
