From 2014.igem.org
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| - | <ul><li></li> | + | <ul><li>PCR'ed out LacI and TetR genes from pAA001 plasmid due to sequence similarity problems</li> |
| - | <li></li> | + | <li>Inserted new pAA001 plasmids into competent cells and plated<\li> |
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| - | <ul><li></li> | + | <ul><li>Picked 12 colonies from yesterday's transformed cells and performed colony PCR</li> |
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Revision as of 22:27, 3 July 2014
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Notebook |
Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
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Week Three |
Monday, 6/30/14
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- Discussion of fallout from Friday's meeting
- More paper-reading to determine what's wrong with our new quorum-sensing system candidates
- Designed geneblocks for our new systems and subgroup projects
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Tuesday, 7/1/14
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- Lab cleanup. Reorganized -20 freezer samples.
- World Cup watching. Tim Howard's a boss
- Work on the team wiki while we wait for our next batch of geneblocks to ship
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Wednesday, 7/2/14
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- PCR'ed out LacI and TetR genes from pAA001 plasmid due to sequence similarity problems
- Inserted new pAA001 plasmids into competent cells and plated<\li>
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Thursday, 7/3/14
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- Picked 12 colonies from yesterday's transformed cells and performed colony PCR
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Friday, 7/4/14
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It's the 4th of July!!!
'Murica...or something like that, I guess...
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"
