Team:TU Darmstadt/Materials/Stock Solution

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Stock Solutions

Antibiotics

Ampicillin

1.Mix

- 4 g ampicillin (100 mg/mL)

- ad. 40 mL ddH2O

2. sterile filtration

3. aliquot in 1 mL stocks and store at -20°C

4. use 1 µL per 1 mL medium

Chloramphenicol

1.Mix

- 1 g chloramphenicol

- ad. 40 mL ethanol

2. aliquot in 1mL stocks and store at -20°C

3. use 1µL per 1mL medium

Kanamycin

1.Mix

- 3 g kanamycin (75 mg/mL)

- ad. 40 mL ddH2O

2.sterile filtration

3.aliquot in 1 mL stocks and store at -20°C

4. use 1µL per 1mL medium

Induction chemicals

IPTG (Isopropyl-beta-D-thiogalactopyranoside)

1. Dissolve 238 mg IPTG in 10 mL water

2. store in 1mL aliquots at -20°C

 

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