Team:Bielefeld-CeBiTec/Project/Isobutanol/Theory

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     <p>The cell uses the products of the carbon dioxide fixation from module II to produce the key metabolite pyruvate. This pyruvate is then used within the third module of our project for further metabolism. Pyruvate is considered as a key metabolite because it can be used as a precursor for different products. We decided to introduce an isobutanol production pathway which starts with pyruvate and is called 2-keto-acid, or Ehrlich, pathway (<a href="#Pamela2012">Pamela P. Peralta-Yahya et al., 2012</a>). For this we want to use and improve existing BioBricks from iGEM Team Formosa 2011/2012.  
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     <p>The aim of the third module of our project is a product synthesis of isobutanol in <i>E. coli</i>. The bacteria should use the products of the carbon dioxide fixation from module II to produce the key metabolite pyruvate. This pyruvate is then used for further metabolism. Pyruvate is considered as a key metabolite because it can be used as a precursor for different industrial relevant products. <br> We decided to introduce an isobutanol production pathway which starts with pyruvate and is called 2-keto-acid, or Ehrlich, pathway (<a href="#Pamela2012">Pamela P. Peralta-Yahya et al., 2012</a>). For this we want to use and improve existing BioBricks from iGEM Team Formosa 2011/2012.  
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Different producing pathways of variable high value products derive from pyruvate. For further purpose the modularity of BioBricks can be used to enable the exchange of those producing systems. Other thinkable products than isobutanol would be isoprene, putrescine or even antibiotics.
Different producing pathways of variable high value products derive from pyruvate. For further purpose the modularity of BioBricks can be used to enable the exchange of those producing systems. Other thinkable products than isobutanol would be isoprene, putrescine or even antibiotics.

Revision as of 22:11, 15 October 2014


Isobutanol

Short summary

The aim of the third module of our project is a product synthesis of isobutanol in E. coli. The bacteria should use the products of the carbon dioxide fixation from module II to produce the key metabolite pyruvate. This pyruvate is then used for further metabolism. Pyruvate is considered as a key metabolite because it can be used as a precursor for different industrial relevant products.
We decided to introduce an isobutanol production pathway which starts with pyruvate and is called 2-keto-acid, or Ehrlich, pathway (Pamela P. Peralta-Yahya et al., 2012). For this we want to use and improve existing BioBricks from iGEM Team Formosa 2011/2012.

Different producing pathways of variable high value products derive from pyruvate. For further purpose the modularity of BioBricks can be used to enable the exchange of those producing systems. Other thinkable products than isobutanol would be isoprene, putrescine or even antibiotics.

Here you will find our results of the isobutanol production.
You can find more information about isobutanol, our genetical approach and an outlook on our wiki

References
  • Pamela P. Peralta-Yahya, Fuzhong Zhang, Stephen B. del Cardayre & Jay D. Keasling, 2012. Microbial engineering for the production of advanced biofuels. In: Nature 488, 320–328

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