Team:Bielefeld-CeBiTec/Project/Isobutanol/GeneticalApproach
From 2014.igem.org
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<h6>Genetical Approach</h6> | <h6>Genetical Approach</h6> | ||
- | <p>There | + | <p>There are different genetical approaches to produce isobutanol with various organisms like cyanobacteria, <i>B. subtilis </i>, <i>E. coli</i> and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012 in <i>E. coli</i>. The iGEM Team Formosa identified the four proteins alsS, ilvC, ilvD and kivD as required for the production of isobutanol in <i>E. coli</i>. We want to reproduce their idea without the temperature shift and with all genes on one plasmid. Additionally we want to combine this plasmid with the adhA from <i>L. lactis</i>.</p> |
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<div class="element" style="width:350px; margin-left:auto; margin-right:auto"> | <div class="element" style="width:350px; margin-left:auto; margin-right:auto"> | ||
<img src="https://static.igem.org/mediawiki/2014/6/67/Bielefeld_CeBiTec_2014-09-01_Isobutanol_weiss_Merck_3.png" width="350px" align="center"> | <img src="https://static.igem.org/mediawiki/2014/6/67/Bielefeld_CeBiTec_2014-09-01_Isobutanol_weiss_Merck_3.png" width="350px" align="center"> | ||
</div> | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="element" style="margin:10px 10px 10px 10px; padding:10px 10px 10px 10px"> | ||
+ | <div id="text"> | ||
+ | <h6>AlsS (α-acetolactate synthase)</h6> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="element" style="margin:10px 10px 10px 10px; padding:10px 10px 10px 10px"> | ||
+ | <div id="text"> | ||
+ | <h6>IlvC (Ketol-acid reductoisomerase)</h6> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="element" style="margin:10px 10px 10px 10px; padding:10px 10px 10px 10px"> | ||
+ | <div id="text"> | ||
+ | <h6>IlvD (Dihydroxyacid dehydratase)</h6> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="element" style="margin:10px 10px 10px 10px; padding:10px 10px 10px 10px"> | ||
+ | <div id="text"> | ||
+ | <h6>KivD (α-ketoisovalerate decarboxylase)</h6> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="element" style="margin:10px 10px 10px 10px; padding:10px 10px 10px 10px"> | ||
+ | <div id="text"> | ||
+ | <h6>AdhA (Alcoholdehydrogenase)</h6> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </body> | ||
+ | </html> |
Revision as of 19:45, 15 October 2014
Isobutanol
Genetical Approach
There are different genetical approaches to produce isobutanol with various organisms like cyanobacteria, B. subtilis , E. coli and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012 in E. coli. The iGEM Team Formosa identified the four proteins alsS, ilvC, ilvD and kivD as required for the production of isobutanol in E. coli. We want to reproduce their idea without the temperature shift and with all genes on one plasmid. Additionally we want to combine this plasmid with the adhA from L. lactis.