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Revision as of 17:21, 15 October 2014

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Organisms

Escherichia coli

Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.

The strain KRX is used for all approaches. Coding sequences of this organism were used for different purposes:

frd - Fumarate reductase
Cytochromes

Domain	Bacteria
Phylum	Proteobacteria
Class	Gammaproteobacteria
Order	Enterobacteriales
Family	Enterobacteriaceae
Genus	Escherichia
Species	E. coli

Lactococcus lactis

Scanning Electron Micrograph of Enterococcus faecalis

Coding sequences of this organism were used for the isobutanol production:

kivD - α-ketoisovalerate decarboxylase
adhA - Alcoholdehydrogenase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Lactobacillales
Family	Streptococcaceae
Genus	Lactococcus
Species	L. lactis

BioBricks created with this organism:

BBa_K1465301
BBa_K1465302
BBa_K1465303
BBa_K1465304
BBa_K1465305
BBa_K1465306
BBa_K1465307

Halothiobacillus neapolitanus

(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.

Coding sequences of this organism were used for the carboxysome:

sap - shell associated protein
can - carbonic anhydrase
csoS1-4 - shell protein
csoS1D - pore protein

Domain	Bacteria
Phylum	Proteobacteria
Class	γ-Proteobacteria
Order	Chromatiales
Family	Halothiobacillaceae
Genus	Halothiobacillus
Species	H. neapolitanus

BioBricks created with this organism:

BBa_K1465202
BBa_K1465204
BBa_K1465205
BBa_K1465206
BBa_K1465207
BBa_K1465208
BBa_K1465209
BBa_K1465213
BBa_K1465214
BBa_K1465222
BBa_K1465223

Bacillus subtilis

The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.

A coding sequence of this organism was used for the isobutanol production:

alsS - α-acetolactate synthase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Bacillales
Family	Bacillaceae
Genus	Bacillus
Species	B. subtilis

BioBricks created with this organism:

BBa_K1465302
BBa_K1465303
BBa_K1465306
BBa_K1465307

Synechococcus elongatus

Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).

A coding sequence of this organism was used for the carbon dioxide fixation:

RuBisCO - Ribulose 1,5-bisphosphate carboxylase / oxygenase
prkA - Phosphoribulokinase

Domain	Bacteria
Phylum	Cyanobacteria
Class	Oscillatoriophycideae
Order	Chroococcales
Genus	Synechococcus
Species	S. elongatus

BioBricks created with this organism:

BBa_K1465201
BBa_K1465203
BBa_K1465212

Actinobacillus succinogenes

Actinobacillus lignieresi. Gram stain.

A coding sequence of this organism was used for the transport of reduction equivalents:

Asuc_1813/1814/1815/1816 - fumarate reductase

Domain	Bacteria
Phylum	Proteobacteria
Class	γ-Proteobacteria
Order	Pasteurellales
Family	Pasteurellaceae
Genus	Actinobacillus
Species	A. succinogenes

BioBricks created with this organism:

BBa_K1465103
BBa_K1465104
BBa_K1465105

Geobacter sulfurreducens

A coding sequence of this organism was used for the transport of electrons:

Cytochromes

Domain	Bacteria
Phylum	Proteobacteria
Subphylum	δ / ε-Proteobacteria
Class	δ-Proteobacteria
Order	Desulfuromonadales
Family	Geobacteraceae
Genus	Geobacter
Species	G. sulfurreducens

BioBricks created with this organism:

BBa_K1465101

Corynebacterium glutamicum

Phase contrast of Corynebacterium glutamicum

A coding sequence of this organism was used for an enzyme assay:

pgk - Phosphoglycerate kinase

Domain	Bacteria
Phylum	Actinobacteria
Class	Actinobacteridae
Order	Actinomycetales
Suborder	Corynebacterineae
Family	Corynebacteriaceae
Genus	Corynebacterium
Species	C. glutamicum

Bacillus methanolicus

Microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.

A coding sequence of this organism was used for carbon dioxide fixation:

glpX - Sedoheptulose 1,7-bisphosphatase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Bacillales
Family	Bacillaceae
Genus	Bacillus
Species	B. methanolicus

BioBricks created with this organism:

BBa_K1465228
BBa_K1465229

References

http://en.wikipedia.org/wiki/Escherichia_coli
http://nl.wikipedia.org/wiki/Melkzuurbacteri
http://en.wikipedia.org/wiki/Carboxysome
http://en.wikipedia.org/wiki/Bacillus_subtilis
http://en.wikipedia.org/wiki/Synechococcus
http://de.wikipedia.org/wiki/Actinobacillus
http://de.wikipedia.org/wiki/Corynebacterium_glutamicum
http://en.wikipedia.org/wiki/Bacillus

@@ Line 149: / Line 149: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465301" target="_blank">BBa_K1465301</a><br><a href="http://parts.igem.org/Part:BBa_K1465302" target="_blank">BBa_K1465302</a><br><a href="http://parts.igem.org/Part:BBa_K1465303" target="_blank">BBa_K1465303</a><br><a href="http://parts.igem.org/Part:BBa_K1465304" target="_blank">BBa_K1465304</a><br><a href="http://parts.igem.org/Part:BBa_K1465305" target="_blank">BBa_K1465305</a><br><a href="http://parts.igem.org/Part:BBa_K1465306" target="_blank">BBa_K1465306</a><br><a href="http://parts.igem.org/Part:BBa_K1465307" target="_blank">BBa_K1465307</a></td>
 </tr>
 </table>
@@ Line 212: / Line 215: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465202" target="_blank">BBa_K1465202</a><br><a href="http://parts.igem.org/Part:BBa_K1465204" target="_blank">BBa_K1465204</a><br><a href="http://parts.igem.org/Part:BBa_K1465205" target="_blank">BBa_K1465205</a><br><a href="http://parts.igem.org/Part:BBa_K1465206" target="_blank">BBa_K1465206</a><br><a href="http://parts.igem.org/Part:BBa_K1465207" target="_blank">BBa_K1465207</a><br><a href="http://parts.igem.org/Part:BBa_K1465208" target="_blank">BBa_K1465208</a><br><a href="http://parts.igem.org/Part:BBa_K1465209" target="_blank">BBa_K1465209</a><br><a href="http://parts.igem.org/Part:BBa_K1465213" target="_blank">BBa_K1465213</a><br><a href="http://parts.igem.org/Part:BBa_K1465214" target="_blank">BBa_K1465214</a><br><a href="http://parts.igem.org/Part:BBa_K1465222" target="_blank">BBa_K1465222</a><br><a href="http://parts.igem.org/Part:BBa_K1465223" target="_blank">BBa_K1465223</a><br></td>
 </tr>
 </table>
@@ Line 272: / Line 278: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465302" target="_blank">BBa_K1465302</a><br><a href="http://parts.igem.org/Part:BBa_K1465303" target="_blank">BBa_K1465303</a><br><a href="http://parts.igem.org/Part:BBa_K1465306" target="_blank">BBa_K1465306</a><br><a href="http://parts.igem.org/Part:BBa_K1465307" target="_blank">BBa_K1465307</a></td>
 </tr>
 </table>
@@ Line 330: / Line 339: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465201" target="_blank">BBa_K1465201</a><br><a href="http://parts.igem.org/Part:BBa_K1465203" target="_blank">BBa_K1465203</a><br><a href="http://parts.igem.org/Part:BBa_K1465212" target="_blank">BBa_K1465212</a></td>
 </tr>
 </table>
@@ Line 390: / Line 402: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465103" target="_blank">BBa_K1465103</a><br><a href="http://parts.igem.org/Part:BBa_K1465104" target="_blank">BBa_K1465104</a><br><a href="http://parts.igem.org/Part:BBa_K1465105" target="_blank">BBa_K1465105</a></td>
 </tr>
 </table>
@@ Line 449: / Line 464: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465101" target="_blank">BBa_K1465101</a></td>
 </tr>
 </table>
@@ Line 619: / Line 637: @@
                 </div>
 </td>
+</tr>
+<tr>
+<td>BioBricks created with this organism:</td><td><a href="http://parts.igem.org/Part:BBa_K1465228" target="_blank">BBa_K1465228</a><br><a href="http://parts.igem.org/Part:BBa_K1465229" target="_blank">BBa_K1465229</a><br></td>
 </tr>
 </table>

Team:Bielefeld-CeBiTec/Notebook/Organisms

From 2014.igem.org

Revision as of 17:21, 15 October 2014

Organisms

References