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Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Oct
From 2014.igem.org
(Difference between revisions)
| Line 86: | Line 86: | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <ul> | ||
| + | <li><b><i>prkA</i></b></li> | ||
| + | <ul> | ||
| + | <li>We tried to bring our <i>prkA</i> with RBS in pSB1C3.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick" target="_blank">BioBrick Assembly</a> (Suffix)</li> | ||
| + | <ul> | ||
| + | <li>Backbone (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Spe</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>)</li> | ||
| + | <ul> | ||
| + | <li>pSB1C3</li> | ||
| + | </ul> | ||
| + | <li>Insert (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Xba</i>I</a>)</li> | ||
| + | <ul> | ||
| + | <li><i>prkA</i></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaheatshock" target="_blank">Transformation</a> with chemocompetent cells</li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of [Construct]</li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Pst</i>I</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>EcoR</i>I</a> </li> | ||
| + | <ul> | ||
| + | <li>Bands as expected (~2000 bp and ~1100 bp)</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | |||
Revision as of 15:27, 15 October 2014
 |
October |
- tkt
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- fba
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- glpX
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- prkA and ptac_Hneap
- We tried to bring the prkA with RBS behind our construct ptac_Hneap but it was not successful.
- prkA
- We tried to bring our prkA with RBS in pSB1C3.
- BioBrick Assembly (Suffix)
- Transformation with chemocompetent cells
- Plasmid isolation of [Construct]
- Restriction digestion with PstI and EcoRI
- Bands as expected (~2000 bp and ~1100 bp)
