Team:Bielefeld-CeBiTec/Project/Isobutanol/Theory

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     <p>The third module benefits from the two modules before. <i>E. coli</i> has gained energy equivalents and reduction equivalents to generate pyruvate by binding carbon dioxide. By using the Calvin cycle the product of our CO2 fixation is pyruvate. This substance is now used as the initial point for the product synthesis. Pyruvate is a starting point of the producing pathways of a variety of high value products like isobutanol, isoprene, putrescine or even antibiotics. Our project implements the producing pathway of isobutanol by using and improving existing BioBricks(iGEM Team Formosa 2011/2012).  
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     <p>The third module benefits from the two modules before. <i>E. coli</i> has gained energy equivalents and reduction equivalents to generate pyruvate by binding carbon dioxide. By using the Calvin cycle the product of our CO2 fixation is pyruvate. This substance is now used as the initial point for the product synthesis. Pyruvate is a starting point of the producing pathways of a variety of high value products like isobutanol, isoprene, putrescine or even antibiotics. Our project implements the producing pathway of isobutanol by using and improving existing BioBricks (iGEM Team Formosa 2011/2012).  
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Revision as of 15:24, 15 October 2014


Isobutanol

Product Synthesis

The third module benefits from the two modules before. E. coli has gained energy equivalents and reduction equivalents to generate pyruvate by binding carbon dioxide. By using the Calvin cycle the product of our CO2 fixation is pyruvate. This substance is now used as the initial point for the product synthesis. Pyruvate is a starting point of the producing pathways of a variety of high value products like isobutanol, isoprene, putrescine or even antibiotics. Our project implements the producing pathway of isobutanol by using and improving existing BioBricks (iGEM Team Formosa 2011/2012).


Figure 1: Schematic illustration of module III
The product synthesis can be changed through the modularity of BioBricks so that variable high value products derived from pyruvate can be implemented in the future.

Isobutanol

Isobutanol is an organic compound. In IUPAC nomenclature it is called 2-methylpropan-1-ol. The advantage of this substance is that it has application fields in many industrial production sites because of this properties. For example it is used in varnish, as an gasoline additive or in paint solvents.
We think that isobutanol is a product which can be used in several ways which makes it interesting as an product of our production system.