Team:Caltech/Notebook

From 2014.igem.org

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     <li>Gel electrophoresis for confirmation of PCR products' length</li>
     <li>Gel electrophoresis for confirmation of PCR products' length</li>
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<ul><li style="list-style-type:circle">With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
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<ul><li>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
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     <li>Meeting with Prof. Murray to discuss give an update on the project</li>
     <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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<ul><li style="list-style-type:circle">Colonies were found and picked after transformation and incubation.</li></ul>
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<ul><li>Colonies were found and picked after transformation and incubation.</li></ul>
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Revision as of 20:26, 1 July 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

Apparently there's a calendar feature to take care of this too???


Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week One

Tuesday, 6/17/14
  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject

Thursday, 6/19/14
  • Formulated plasmid design (pAA001) for the response regulation subproject
  • PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
  • With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
  • Colonies were found and picked after transformation and incubation.


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