Team:Caltech/Notebook

From 2014.igem.org

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<br><br>
<b><a href='Overview.html'>Overview</a></b><br><br>
<b><a href='Overview.html'>Overview</a></b><br><br>
<b><a href='week1.html'>Week 1</a></b><br><br>
<b><a href='week1.html'>Week 1</a></b><br><br>
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<b><a href='week12.html'>Week 12</a></b><br><br>
<b><a href='week12.html'>Week 12</a></b><br><br>
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<table border=1>
<table border=1>
<tr><td colspan=3><h1>Week One</h1></td></tr>
<tr><td colspan=3><h1>Week One</h1></td></tr>
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<tr><td width=20% valign=top>Tuesday, 6/17</td>
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     <td width=80% valign=top> put in the crap here</td>
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<h4>Tuesday, 6/17/14</h4>
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<ul><li>Get acquainted with summer logistics and lab basics</li>
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     <li>Clean up lab space, stock lab supplies</li>
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</ul>
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<td width="25%valign="top">  
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<h4>Wednesday, 6/18/14</h4>
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<ul><li>Continue to discuss project and clean up lab space</li>
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    <li>Designed PCR primers for combinatorial promoters subproject</li>
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</ul>
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<h4>Thursday, 6/19/14</h4>
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<ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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    <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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    <li>Gel electrophoresis for confirmation of PCR products' length</li>
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</ul>
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<ul><li style="list-style-type:circle">With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
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<h4>Friday, 6/20/14</h4>
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<ul><li>Purification of PCR products created yesterday</li>
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    <li>Gibson assembly of purified products</li>
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    <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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    <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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</ul>
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<ul><li style="list-style-type:circle">Colonies were found and picked after transformation and incubation.</li></ul>
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</table>
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Revision as of 20:25, 1 July 2014


WELCOME TO iGEM 2014!

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Notebook

Apparently there's a calendar feature to take care of this too???


Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week One

Tuesday, 6/17/14

  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies

Wednesday, 6/18/14

  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject

Thursday, 6/19/14

  • Formulated plasmid design (pAA001) for the response regulation subproject
  • PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
  • With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14

  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
  • Colonies were found and picked after transformation and incubation.