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| | <tr><td colspan="3"> <h3>Notebook</h3> Apparently there's a calendar feature to take care of this too???</td></tr> | | <tr><td colspan="3"> <h3>Notebook</h3> Apparently there's a calendar feature to take care of this too???</td></tr> |
| | </tr> | | </tr> |
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| - | <tr><td colspan=3><h1>Week One</h1></td></tr>
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| | <tr> | | <tr> |
| - | <td width="20%" valign="top"> | + | <td> |
| - | <h4>Tuesday, 6/17/14</h4> | + | <!-- insert the links here --> |
| | + | <b><a href='Overview.html'>Overview</a></b><br> |
| | + | <b><a href='week1.html'>Week 1</a></b><br> |
| | + | <b><a href='week2.html'>Week 2</a></b><br> |
| | + | <b><a href='week3.html'>Week 3</a></b><br> |
| | + | <b><a href='week4.html'>Week 4</a></b><br> |
| | + | <b><a href='week5.html'>Week 5</a></b><br> |
| | + | <b><a href='week6.html'>Week 6</a></b><br> |
| | + | <b><a href='week7.html'>Week 7</a></b><br> |
| | + | <b><a href='week8.html'>Week 8</a></b><br> |
| | + | <b><a href='week9.html'>Week 9</a></b><br> |
| | + | <b><a href='week10.html'>Week 10</a></b><br> |
| | + | <b><a href='week11.html'>Week 11</a></b><br> |
| | + | <b><a href='week12.html'>Week 12</a></b><br> |
| | </td> | | </td> |
| - | <td width="40%" valign="top"> | + | <td> |
| - | <ul><li>Get acquainted with summer logistics and lab basics</li> | + | <table valign=top> |
| - | <li>Clean up lab space, stock lab supplies</li>
| + | <tr><td colspan=3><h1>Week One</h1></td></tr> |
| - | </ul> | + | <tr><td width=20%>Tuesday, 6/17</td> |
| - | </td> | + | <td width=80%> put in the crap here</td> |
| - | <td>We got absolutely no results today!!!</td> | + | |
| | </tr> | | </tr> |
| - | | + | </table> |
| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Wednesday, 6/18/14</h4>
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| | </td> | | </td> |
| - | <td width="40%" valign="top">
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| - | <ul><li>Continue to discuss project and clean up lab space</li>
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| - | <li>Designed PCR primers for combinatorial promoters subproject</li>
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| - | </ul>
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| - | </td>
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| - | <td>We got absolutely no results today!!!</td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Thursday, 6/19/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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| - | <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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| - | <li>Gel electrophoresis for confirmation of PCR products' length</li>
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| - | </ul>
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| - | </td>
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| - | <td>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Friday, 6/20/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Purification of PCR products created yesterday</li>
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| - | <li>Gibson assembly of purified products</li>
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| - | <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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| - | <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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| - | </ul>
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| - | </td>
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| - | <td>Colonies were found and picked after transformation and incubation.</td>
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| - | </tr>
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| - | <tr><td colspan=3><h1>Week Two</h1></td></tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Monday, 6/23/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Discussion of fallout from Friday's meeting, paper reading</li>
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| - | <li>Colony PCR and Minipreps of liquid cultures of colonies picked over the weekend</li>
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| - | <li>TXTL workshop starts today</li>
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| - | </ul>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Tuesday, 6/24/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Discussed alternatives to current ComQXPA system, including 2-cell system (not all in 1 cell)</li>
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| - | <li>Gibson assembly of another combinatorial promoter using pKS001 backbone, lacI geneblock, ____ promoter geneblock, and sfGFP geneblock.</li>
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| - | <li>Transformation of Gibson constructs into JM109 cells</li>
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| - | <li>Linear combinatorial constructs created via PCR of pAA001 (minipreps) to be tested in TXTL</li>
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| - | </ul>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Wednesday, 6/25/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Linear constructs and plasmid TXTL reactions of A90 promoter (pAA001) were set up and run</li>
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| - | <li>Gibson assembly of plasmid pAA002 containing B83 promoter</li>
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| - | <li>PCR of Gibson assemblies of pAA002 to create linear constructs to test in TXTL</li>
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| - | <li>TXTL reactions of B83 promoter (linear frag. from pAA002) were set up and run</li>
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| - | <li>Transformation of pAA002 Gibson assemblies into JM109</li>
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| - | </ul>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Thursday, 6/26/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Analysis of yesterday's TXTL reactions (run overnight)</li>
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| - | <li>Colony PCR and miniprep of bacterial colonies transformed with pAA002. Minipreps shipped for sequencing.</li>
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| - | </ul>
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| - | </td>
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| - | <td>TXTL reaction data inconclusive. Most fluorescence signals are below negative control, and linear data frequently contradicts plasmid data with A90 promoter. No signal observed for B83 promoter.</td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%" valign="top">
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| - | <h4>Friday, 6/27/14</h4>
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| - | </td>
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| - | <td width="40%" valign="top">
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| - | <ul><li>Continued to investigate different signalling pathways</li>
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| - | <li>Potential alternative pathways to investigate:
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| - | <ul><li>fsrABCD</li><li>agrBDCA</li><li>uhpABCT</li></ul></li>
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| - | </ul>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| | </table> | | </table> |
| | <br><br> | | <br><br> |
| | </html> | | </html> |
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