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Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Oct
From 2014.igem.org
(Difference between revisions)
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<div class="content" style="margin-right:10%; margin-left:10%"> | <div class="content" style="margin-right:10%; margin-left:10%"> | ||
<ul> | <ul> | ||
| - | <li><b>tkt</b></li> | + | <li><b><i>tkt</i></b></li> |
<ul> | <ul> | ||
<li>This week we wanted to do the enzyme assay for the SBPase.</li> | <li>This week we wanted to do the enzyme assay for the SBPase.</li> | ||
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</ul> | </ul> | ||
</ul> | </ul> | ||
| + | <br> | ||
<ul> | <ul> | ||
| - | <li><b>fba</b></li> | + | <li><b><i>fba</i></b></li> |
<ul> | <ul> | ||
<li>This week we wanted to do the enzyme assay for the SBPase.</li> | <li>This week we wanted to do the enzyme assay for the SBPase.</li> | ||
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</ul> | </ul> | ||
</ul> | </ul> | ||
| + | <br> | ||
<ul> | <ul> | ||
| - | <li><b>glpX</b></li> | + | <li><b><i>glpX</i></b></li> |
<ul> | <ul> | ||
<li>This week we wanted to do the enzyme assay for the SBPase.</li> | <li>This week we wanted to do the enzyme assay for the SBPase.</li> | ||
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</ul> | </ul> | ||
</ul> | </ul> | ||
| + | <br> | ||
| + | |||
| + | <ul> | ||
| + | <li><b><i>prkA</i> and p<sub>tac</sub>_Hneap</b></li> | ||
| + | <ul> | ||
| + | <li>We tried to bring the <i>prkA</i> with RBS behind our construct p<sub>tac</sub>_Hneap but it was not successful.</li> | ||
| + | </ul> | ||
| + | </ul> | ||
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</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:15, 15 October 2014
 |
October |
- tkt
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- fba
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- glpX
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- prkA and ptac_Hneap
- We tried to bring the prkA with RBS behind our construct ptac_Hneap but it was not successful.
