Team:UESTC-China/result
From 2014.igem.org
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<div ><img style="width:450px;height:280px ;" src="https://static.igem.org/mediawiki/2014/c/cf/Rrfig1.JPG"></div> | <div ><img style="width:450px;height:280px ;" src="https://static.igem.org/mediawiki/2014/c/cf/Rrfig1.JPG"></div> | ||
- | <div ><p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <div ><p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.1</strong> Verification of backbones using digestion | <strong>Fig.1</strong> Verification of backbones using digestion | ||
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<div align="center"><img style="width:300px;height:280px ;" src="https://static.igem.org/mediawiki/2014/3/30/R3.JPG"> | <div align="center"><img style="width:300px;height:280px ;" src="https://static.igem.org/mediawiki/2014/3/30/R3.JPG"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.2</strong> | <strong>Fig.2</strong> | ||
Digestion the plasmid piGEM005 with EcoRI and SacI. | Digestion the plasmid piGEM005 with EcoRI and SacI. | ||
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<div align="center"><img style="width:550px;height:280px ;" src="https://static.igem.org/mediawiki/2014/7/7b/Rr2.JPG"> | <div align="center"><img style="width:550px;height:280px ;" src="https://static.igem.org/mediawiki/2014/7/7b/Rr2.JPG"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.3</strong> Verification of multi-gene expression vectors using digestion | <strong>Fig.3</strong> Verification of multi-gene expression vectors using digestion | ||
<strong>A.</strong>Digestion the plasmid piGEM009 with XbaI and SalI. | <strong>A.</strong>Digestion the plasmid piGEM009 with XbaI and SalI. | ||
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<div align="center"><img style="width:60% ;" src="https://static.igem.org/mediawiki/2014/7/75/Rr4.JPG"> | <div align="center"><img style="width:60% ;" src="https://static.igem.org/mediawiki/2014/7/75/Rr4.JPG"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:700px; color:#1b1b1b;"> |
<strong>Fig.4</strong> | <strong>Fig.4</strong> | ||
Transform piGEM003, piGEM004, piGEM005, piGEM006, piGEM007, piGEM008, piGEM009, piGEM010 and piGEM011 into tobacco. Co-cultured for 48 hours(A); Screening cultivation for one month(B); Rooting cultivation for one month(C). | Transform piGEM003, piGEM004, piGEM005, piGEM006, piGEM007, piGEM008, piGEM009, piGEM010 and piGEM011 into tobacco. Co-cultured for 48 hours(A); Screening cultivation for one month(B); Rooting cultivation for one month(C). | ||
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<div align="center"> | <div align="center"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"><strong>Table.1</strong> |
Statistical result of quantity of each transgenic line. | Statistical result of quantity of each transgenic line. | ||
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<div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/5/58/10.png"> | <div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/5/58/10.png"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"><strong>Fig.5</strong> |
PCR identification of kan-resistant tobacco seedlings (piGEM010 transgenic line). | PCR identification of kan-resistant tobacco seedlings (piGEM010 transgenic line). | ||
M: DNA marker; | M: DNA marker; | ||
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<div align="center"><img style="width:45% ;" src="https://static.igem.org/mediawiki/2014/4/43/RrRNA.png"> | <div align="center"><img style="width:45% ;" src="https://static.igem.org/mediawiki/2014/4/43/RrRNA.png"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.6</strong>RT-PCR verification of positive transgenic tobacco seedlings.<br/> | <strong>Fig.6</strong>RT-PCR verification of positive transgenic tobacco seedlings.<br/> | ||
M: DNA marker; 1-8: 6 individual lines; 9: plasmid | M: DNA marker; 1-8: 6 individual lines; 9: plasmid | ||
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<div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/9/9e/12.png"> | <div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/9/9e/12.png"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.7</strong> | <strong>Fig.7</strong> | ||
The transgenic plants (A) and wildtype(B), which had been grown separately in sealed boxes, were exposed to HCHO evaporated from a micro tube (0.5ml) containing HCHO solution (37%,50ul) | The transgenic plants (A) and wildtype(B), which had been grown separately in sealed boxes, were exposed to HCHO evaporated from a micro tube (0.5ml) containing HCHO solution (37%,50ul) | ||
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<div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/c/c0/13.png"> | <div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/c/c0/13.png"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.8</strong> | <strong>Fig.8</strong> | ||
Phenotype testing of transgenetic seedlings and wildtype. A: Before exposure to HCHO. B: Exposure to HCHO for one week. The transgenetic seedling is stronger than wildtype after formaldehyde exposure. 20ul 37% HCHO, one week. | Phenotype testing of transgenetic seedlings and wildtype. A: Before exposure to HCHO. B: Exposure to HCHO for one week. The transgenetic seedling is stronger than wildtype after formaldehyde exposure. 20ul 37% HCHO, one week. | ||
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<div align="center"><img style="width:10% ;" src="#"> | <div align="center"><img style="width:10% ;" src="#"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"> |
<strong>Fig.9</strong> | <strong>Fig.9</strong> | ||
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<div align="center"><img style="width:35% ;" src="https://static.igem.org/mediawiki/2014/0/00/R13.JPG"> | <div align="center"><img style="width:35% ;" src="https://static.igem.org/mediawiki/2014/0/00/R13.JPG"> | ||
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align: | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:650px; color:#1b1b1b;"><strong>Fig.10</strong> |
Gaseous HCHO tolerance and uptake capacity of transgenic tobacco plants (<i>Li-Men Chen et, al. 2009</i>) | Gaseous HCHO tolerance and uptake capacity of transgenic tobacco plants (<i>Li-Men Chen et, al. 2009</i>) | ||
</p> | </p> |
Revision as of 12:57, 15 October 2014