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Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep
From 2014.igem.org
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| + | <ul> | ||
| + | <li><b><i>pSB1K3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></i></b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to reclone <i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> into pSB1C3 </li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> of pSB1K3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> and pSB1C3_<i>RFP</i> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>EcoR</i>I</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Pst</i>I</a> </li> | ||
| + | <ul> | ||
| + | <li>Bands as expected <br>(~ 7500 bp for <i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> and ~ 2200 bp for pSB1C3)</li> | ||
| + | </ul><li>Clean up and Ligation as explained in the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick" target="_blank">BioBrick Assembly</a></li> | ||
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Revision as of 10:48, 15 October 2014
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September |
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- adhA
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1K3_adhA
- Restriction digestion with EcoRI and PstI of pSB1K3_adhA
- Bands as expected (~1200 bp)
- Successful sequencing
Agarose gel from the restriction digestion with EcoRI and PstI. As a Ladder we used GeneRuler™ 1 kb DNA Ladder from Thermo Scientific.
- T7_alsS_ilvC_ilvD_kivD
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Restriction digestion with EcoRI and PstI of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Bands not as expected (~7000bp)
- pSB1K3_alsS_ilvC_ilcD_kivD_adhA
- This week we tried to combine the pSB1K3_alsS_ilvC_ilvD_kivD with adhA
- BioBrick Assembly (Suffix)
- Colony PCR (fw-ilvD-kivD, rev-pSB1C3-adhA)
- Annealing temperature: 65 °C
- Bands not as expected (~ 3800 bp)
- pSB1K3_alsS_ilvC_ilvD_kivD
- This week we tried to reclone alsS_ilvC_ilvD_kivD into pSB1C3
- Restriction digestion of pSB1K3_alsS_ilvC_ilvD_kivD and pSB1C3_RFP with EcoRI and PstI
- Bands as expected
(~ 7500 bp for alsS_ilvC_ilvD_kivD and ~ 2200 bp for pSB1C3) - Clean up and Ligation as explained in the BioBrick Assembly
