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Team:CityU HK/project/result
From 2014.igem.org
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<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2> | <h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2> | ||
| - | <p class="content">Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).<br> | + | <p class="content">Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472606</a>).<br> |
Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.<br> | Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.<br> | ||
| - | Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).<br> | + | Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a>).<br> |
Successfully constructed a translational unit of the ‘tesA gene in pAB1A2. <br> | Successfully constructed a translational unit of the ‘tesA gene in pAB1A2. <br> | ||
Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.<br> | Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.<br> | ||
| - | Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).<br> | + | Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a>).<br> |
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br> | Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br> | ||
Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br> | Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br> | ||
Revision as of 08:38, 15 October 2014
Result Summary
Experimental Achievements from 15 June – 3 October 2014 :
Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).
Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.
Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).
Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.
Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.
Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.
Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
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