Team:Aachen/Notebook/Engineering/WatsOn

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Revision as of 06:53, 15 October 2014

Software

The software consists of several parts which provide a user interface and manage the connection to the hardware.

Different components of the software:

GUI : with the graphical interface the user can take images and time lapse of the chips in the device. The software is written in C++. It makes use of the Qt-Library[http://qt-project.org/] to provide a clear interface and a comfortable way to manage various software aspects such as handling images and establishing network connections. An advantage resulting from the utilization of Qt-Library is the multi-platform support for Windows, MacOS and Linux. Additionally Qt is available with an Open-source license which can be used for free. The software can be used locally on the Raspberry Pi or remotely from a device in the same network.
Features:
- change settings:
  - the user can specify the iso-value and the shutter speed of the camera
  - custom settings can be labeled and saved for future usage
  - existing settings can be updated or deleted unless they are no default configurations
  - the excitation of the LEDs for GFP(480nm) and iLOV(450nm) can be selected
- take image/s:
  - the GUI offers two possibilities to take images:
    - take single image with the active camera setting
    - take time lapse shootings with the active camera settings and the specified interval. When activated the images are saved automatically to a user defined directory with ascending filenames
  - the last image which was taken by the camera is shown in the GUI, information containing the timestamp and used camera settings are displayed next to the image. Previous images can be selected with the arrow buttons.
- analyse image
  - the image is analysed by an image segmentation algorithm and shows whether the pathogen Pseudomonas aeruginosa is present on the chip or not

Backend: The backend is a software that runs on the Raspberry Pi and is responsible for the connection between the GUI and the hardware. If the user interface is executed on another device e.g. a notebook it has to be in the same network as the Raspberry Pi. The backend works like a webserver that receives commands and acts corresponding to the submitted parameters. It can take images and returns them to the GUI.

Before an image is taken the backend turns on the specified LEDs by sending a command to the connected Arduino board. Afterwards the LEDs are turned off using the same mechanism. These steps are repeated in the given interval for a time lapse shooting.

Hardware

Raspberry Pi :

Arduino: The arduino board sets the current and thus the temperature of the Peltier heater by evaluating the received values from the temperature sensors for the interior and the aluminium block. Additionally it receives commands from the Raspberry Pi to turn the LEDs on and off.

Build your own WatsOn

If you want to create your own WatsOn at first check the following list of necessary devices. All needed components can be ordered by iusing the internet or local provider. Just for cutting the acryllic glass in the right form is more complicated. Therefore a laser cutting method is the best way which is offer by [http://www.formulor.de/ formulor].

Table 1: Needed number of pieces, components and material costs for building your own measurement device

number of pieces	components	costs [$]
1	[http://www.prolighting.de/Zubehoer/Farbfilter/Lee-Filter_HT/Lee-Filters_Musterheft_Designer_Edition_i174_3965_0.htm filter slides] (medium yellow 010, sally green 505)	5.17
1	[http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600 arduino UNO R3]	11.65
1	[http://www.dx.com/p/arduino-2-channel-relay-shield-module-red-144140 2-channel relay shield]	3.46
40	jumper-wire cable	2.99
1	[http://www.dx.com/p/2-54mm-1x40-pin-breakaway-straight-male-header-10-piece-pack-144191 40er male header (10-Piece Pack)]	2.72
1	[http://www.dx.com/p/jtron-2-54mm-40-pin-single-row-seat-single-row-female-header-black-10-pcs-278953 40er female header (10-Piece Pack)]	2.60
1	[http://www.dx.com/p/prototype-universal-printed-circuit-board-breadboard-brown-5-piece-pack-130926 circuit board]	2.99
1	[http://www.newark.com/pro-signal/rp006/audio-video-cable-hdmi-1m-black/dp/96T7446 HDMI cable]	1.87
1	[http://www.dx.com/p/hd-053-high-speed-usb-2-0-7-port-hub-black-174817 7 port USB hub]	6.71
1	[http://www.dx.com/p/dx-original-ultra-mini-usb-2-0-802-11n-b-g-150mbps-wi-fi-wlan-wireless-network-adapter-black-252716 WLAN USB stick]	5.35
1	USB mouse and keyboard	12.50
1	[http://corporate.evonik.com/en/products/pages/default.aspx case acrylic glass XT 6mm~0.5²]	50.65
1	spray paint for acrylic glass	6.54
1	[http://www.newark.com/raspberry-pi/raspberry-modb-512m/raspberry-pi-model-b-board/dp/68X0155 Raspberry Pi model B board]	35.00
1	[http://www.newark.com/raspberry-pi/rpi-camera-board/add-on-brd-camera-module-raspberry/dp/69W0689 Raspberry Pi camera module]	25.00
1	[http://www.pollin.de/shop/dt/NzUwOTc4OTk-/ 7” display]	50.50
1	[http://www.dx.com/p/diy8-x-seven-segment-displays-module-for-arduino-595-driver-250813 8-segment display]	3.86
2	[http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531 digital temperature sensor DHT-22]	7.50
1	aluminum block 100x100x15 mm	14.23
1	[http://www.dx.com/p/tec1-12706-semiconductor-thermoelectric-cooler-peltier-white-157283 Peltier heater 12V 60W]	4.49
1	power supply	32.89
6	[http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html superflux LED 480nm]	7.50
16	LED 450nm	7.50
2	Resistor 40 Ohm	0.30
4	Resistor 100 Ohm	0.60
1	cupboard button	1.24
-	total	309.45

You can find more economical information for WatsOn and the project on our Economical View page.

Wiring of our device

	Start building your own WatsOn by assembling the base plate, the sides and the interior wall.
	Fix the Peltier heater on the back of the aluminum block and place it in the hole of the interior wall. Arrange the 4x4 450nm LEDs and the 2x3 480nm LEDs
	Assemble the camera holder with the camera and the corresponding filter slide on the lower part. Over the camera you can place the temperature sensor for measuring the indoor temperature. Finally put the fan on the back of the camera holder.
	Connect the electronic components on the outside and the inside according to the wiring diagramm.
	Put together the drawer.
	Position the front panel and insert the drawer.
	Place the temperature sensor for the aluminium block temperature directly on the block and put the back panel in front of it.
	Setup the power supply and connect all devices to either 5V or 12V.
	Mount the device on top of the power supply casing. Add the display and apply some stickers to enjoy your custom-made WatsOn.

Contact Disclaimer

@@ Line 26: / Line 26: @@
 * '''Backend''': The backend is a software that runs on the Raspberry Pi and is responsible for the connection between the GUI and the hardware. If the user interface is executed on another device e.g. a notebook it has to be in the same network as the Raspberry Pi. The backend works like a webserver that receives commands and acts corresponding to the submitted parameters. It can take images and returns them to the GUI.
 Before an image is taken the backend turns on the specified LEDs by sending a command to the connected Arduino board. Afterwards the LEDs are turned off using the same mechanism. These steps are repeated in the given interval for a time lapse shooting.
@@ Line 36: / Line 35: @@
 * '''Arduino''': The arduino board sets the current and thus the temperature of the Peltier heater by evaluating the received values from the temperature sensors for the interior and the aluminium block. Additionally it receives commands from the Raspberry Pi to turn the LEDs on and off.
+{{Team:Aachen/BlockSeparator}}