Team:Aachen/Project/Measurement Device

From 2014.igem.org

(Difference between revisions)
(WatsOn)
Line 35: Line 35:
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Measure-<br/>ments</div></div>
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Measure-<br/>ments</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
 +
    </div>
 +
    </a>
 +
  </li>
 +
 +
  <li>
 +
    <a class="menulink" href="https://2014.igem.org/Team:Aachen/PolicyPractices/Economics#economicswatson" style="color:black">
 +
    <div class="menusmall-item menusmall-info" ><div class="menukachel">Economical View</div></div>
 +
    <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
     </a>
     </a>
Line 42: Line 50:
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonsecretingredients" style="color:black">
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonsecretingredients" style="color:black">
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Secret<br/>Ingredients</div></div>
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Secret<br/>Ingredients</div></div>
 +
    <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
 +
    </div>
 +
    </a>
 +
  </li>
 +
 +
  <li>
 +
    <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn" style="color:black">
 +
    <div class="menusmall-item menusmall-info" ><div class="menukachel">DIY</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
Line 108: Line 124:
{{Team:Aachen/FigureFloatRight|Aachen_Device_11.jpg|title= |subtitle= |width=330px}}
{{Team:Aachen/FigureFloatRight|Aachen_Device_11.jpg|title= |subtitle= |width=330px}}
''Awaiting synthesis...''
''Awaiting synthesis...''
-
 
-
{{Team:Aachen/BlockSeparator}}
 
-
 
-
== Build your own ''WatsOn'' ==
 
-
 
-
---> Move to "Engineering"
 
-
 
-
If you want to create your own ''WatsOn'' at first check the following list of necessary devices.
 
-
 
-
<center>
 
-
'''Table 1''': Needed number of pieces, components and costs for building your own measurement device
 
-
</center>
 
-
{| class="wikitable"
 
-
! number of pieces !! components !! costs [$]
 
-
|-
 
-
| 1|| [http://www.prolighting.de/Zubehoer/Farbfilter/Lee-Filter_HT/Lee-Filters_Musterheft_Designer_Edition_i174_3965_0.htm filter slides] (medium yellow 010, sally green 505)||style="text-align:right" |5.17
 
-
|-
 
-
| 1|| [http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600 arduino UNO R3]||style="text-align:right" |11.65
 
-
|-
 
-
| 1|| [http://www.dx.com/p/arduino-2-channel-relay-shield-module-red-144140 2-channel relay shield]||style="text-align:right" |3.46
 
-
|-
 
-
| 40||jumper-wire cable||style="text-align:right" |2.99
 
-
|-
 
-
| 1|| [http://www.dx.com/p/2-54mm-1x40-pin-breakaway-straight-male-header-10-piece-pack-144191 40er male header (10-Piece Pack)]||style="text-align:right" |2.72
 
-
|-
 
-
| 1|| [http://www.dx.com/p/jtron-2-54mm-40-pin-single-row-seat-single-row-female-header-black-10-pcs-278953 40er female header (10-Piece Pack)]||style="text-align:right" |2.60
 
-
|-
 
-
| 1|| [http://www.dx.com/p/prototype-universal-printed-circuit-board-breadboard-brown-5-piece-pack-130926 circuit board]||style="text-align:right" |2.99
 
-
|-
 
-
| 1|| [http://www.newark.com/pro-signal/rp006/audio-video-cable-hdmi-1m-black/dp/96T7446 HDMI cable]||style="text-align:right" |1.87
 
-
|-
 
-
| 1|| [http://www.dx.com/p/hd-053-high-speed-usb-2-0-7-port-hub-black-174817 7 port USB hub]||style="text-align:right" |6.71
 
-
|-
 
-
| 1||[http://www.dx.com/p/dx-original-ultra-mini-usb-2-0-802-11n-b-g-150mbps-wi-fi-wlan-wireless-network-adapter-black-252716 WLAN USB stick]||style="text-align:right" |5.35
 
-
|-
 
-
| 1||USB mouse and keyboard||style="text-align:right" |12.50
 
-
|-
 
-
| 1|| [http://corporate.evonik.com/en/products/pages/default.aspx case acrylic glass XT 6mm~0.5<sup>2</sup>]||style="text-align:right" |50.65
 
-
|-
 
-
| 1||spray paint for acrylic glass||style="text-align:right" |6.54
 
-
|-
 
-
| 1|| [http://www.newark.com/raspberry-pi/raspberry-modb-512m/raspberry-pi-model-b-board/dp/68X0155 Raspberry Pi model B board]||style="text-align:right" |35.00
 
-
|-
 
-
| 1||[http://www.newark.com/raspberry-pi/rpi-camera-board/add-on-brd-camera-module-raspberry/dp/69W0689 Raspberry Pi camera module]||style="text-align:right" |25.00
 
-
|-
 
-
| 1||[http://www.pollin.de/shop/dt/NzUwOTc4OTk-/ 7” display]||style="text-align:right" |50.50
 
-
|-
 
-
| 1||[http://www.dx.com/p/diy8-x-seven-segment-displays-module-for-arduino-595-driver-250813 8-segment display]||style="text-align:right" |3.86
 
-
|-
 
-
| 2|| [http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531 digital temperature sensor DHT-22]||style="text-align:right" |7.50
 
-
|-
 
-
| 1 ||aluminum block 100x100x15 mm||style="text-align:right" |14.23
 
-
|-
 
-
| 1|| [http://www.dx.com/p/tec1-12706-semiconductor-thermoelectric-cooler-peltier-white-157283 Peltier heater 12V 60W]||style="text-align:right" |4.49
 
-
|-
 
-
| 1||power supply||style="text-align:right" |32.89
 
-
|-
 
-
| 6|| [http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html superflux LED 480nm]||style="text-align:right" |7.50
 
-
|-
 
-
| 16||LED 450nm||style="text-align:right" |7.50
 
-
|-
 
-
| 2|| Resistor 40 Ohm||style="text-align:right" |0.30
 
-
|-
 
-
| 4|| Resistor 100 Ohm||style="text-align:right" |0.60
 
-
|-
 
-
| 1||cupboard button||style="text-align:right" |1.24
 
-
|- style="border-top: 2px #808080 solid; background: #cfe2f4; font-weight: bold;"
 
-
| -||total||style="text-align:right" |309.45
 
-
|}
 
-
 
-
You can find more economical information for ''WatsOn'' and the project on our [https://2014.igem.org/Team:Aachen/PolicyPractices/Economics Economical View] page.
 
-
 
-
{{Team:Aachen/Figure|Aachen_Device_Fritzing.png|align=center|title=Wiring of our device||width=900px}}
 
-
 
-
{| class="wikitable centered"
 
-
|-
 
-
| [[File:Aachen_Device_1.jpg|300px]] || Start building your own ''WatsOn'' by assembling the base plate, the sides and the interior wall.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_2_3.jpg|350px] [File:Aachen_Device_.3jpg|300px]] || Fix the Peltier heater on the back of the aluminum block and place it in the hole of the interior wall.<html><br/></html>Arrange the 4x4 450nm LEDs and the 2x3 480nm LEDs
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_7.jpg|350px]] || Assemble the camera holder with the camera and the corresponding filter slide on the lower part. Over the camera you can place the temperature sensor for measuring the indoor temperature. Finally put the fan on the back of the camera holder.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_8.jpg|350px]] || Connect the electronic components on the outside and the inside according to the wiring diagramm.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_4.jpg|350px]] || Put together the drawer.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_9.jpg|350px]] || Position the front panel and insert the drawer.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_10.jpg|350px]] || Place the temperature sensor for the aluminium block temperature directly on the block and put the back panel in front of it.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_6.jpg|350px]] || Setup the power supply and connect all devices to either 5V or 12V.
 
-
|-style="border-top: 2px #808080 solid;"
 
-
| [[File:Aachen_Device_11.jpg|350px]] || Mount the device on top of the power supply casing. Add the display and apply some stickers to enjoy your custom-made ''WatsOn''.
 
-
|}
 
{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Revision as of 17:06, 14 October 2014

WatsOn

Part of the biosensor system Cellock Holmes are our agar chips. They contain our engineered sensing cells that fluoresce in the presence of the pathogen Pseudomonas aeruginosa. However, bacteria are small and the fluorescent signal cannot be seen with the naked eye. Thus the central question "What's on the chip?" remains.

To answer this question we present our measurement device WatsOn.

Functions

WatsOn fulfills several tasks in our system:

  • incubate the sensing cells and the sampling chip
  • prevent escape of potentially sampled pathogens and our genetically engineered cells
  • illuminate the chip with the right excitation wavelength for GFP or iLOV
  • take photos and timeseries of the chips
  • analyze the fluorescence signal
  • give feedback to the user about the presence or absence of P. aeruginosa

WatsOn is designed such that it can be easily copied. Our work heavily emphasizes the Open Source concept. Therefore, the construction manual and all technical detailes are published on our wiki. Analogous to our OD/F device, we used low-cost and easily available parts.

Measurarty

Awaiting synthesis...

Software Evaluation

Awaiting synthesis...

Measurements

Awaiting synthesis...


Secret Ingredients

Awaiting synthesis... Arduino Sketchbook (.ino)

Achievements

Aachen Device 11.jpg
'

Awaiting synthesis...