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Team:UiOslo Norway/Project
From 2014.igem.org
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<h3>Blabla.</h3> | <h3>Blabla.</h3> | ||
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis ab sit exercitationem iste culpa neque tempore accusantium nobis asperiores dicta, sunt vitae architecto adipisci, enim minus esse atque. Ab maxime ad numquam praesentium, est repellendus possimus, nam et, enim, ea quibusdam. Libero unde sunt nihil quibusdam dolorum iste quasi blanditiis quod soluta voluptates illum eaque temporibus, mollitia molestias, illo ratione dolore velit commodi quae at natus possimus ab. Molestiae sint quisquam unde provident magni, reprehenderit pariatur at facilis ipsam illum error, dignissimos ad temporibus, incidunt doloremque? Reiciendis voluptates facilis, eius dolores ab inventore, ducimus veniam eligendi. Impedit nemo accusamus unde!</p> | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis ab sit exercitationem iste culpa neque tempore accusantium nobis asperiores dicta, sunt vitae architecto adipisci, enim minus esse atque. Ab maxime ad numquam praesentium, est repellendus possimus, nam et, enim, ea quibusdam. Libero unde sunt nihil quibusdam dolorum iste quasi blanditiis quod soluta voluptates illum eaque temporibus, mollitia molestias, illo ratione dolore velit commodi quae at natus possimus ab. Molestiae sint quisquam unde provident magni, reprehenderit pariatur at facilis ipsam illum error, dignissimos ad temporibus, incidunt doloremque? Reiciendis voluptates facilis, eius dolores ab inventore, ducimus veniam eligendi. Impedit nemo accusamus unde!</p> | ||
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| + | <p><strong>Our Projects</strong></p> | ||
| + | <p>In order to make a MicroOrganizer, we needed to find a enzyme that could be split in two parts, and restore its function when the parts meet. We chose β-galactosidase gene, LacZ. β-galactosidase can be split into two parts β-galactosidase-α and β-galactosidase-β (Ullman et al. 1967). The functional enzyme consisting of one alpha and one beta part hydrolyses lactose into galactose and glucose. This process produces on some occasions allolactose which induces the Lac-operon. This connection between the enzyme product and the Lac operon provides the needed signalling mechanism. We have isolated some different autotransporters and have not yet decided which to use.</p> | ||
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| + | <p><strong>References</strong></p> | ||
| + | <p>Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli. | ||
| + | Ullmann A, Jacob F, Monod J | ||
| + | J Mol Biol. 1967 Mar 14; 24(2):339-43.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:41, 14 October 2014
Project Overview
Blabla.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis ab sit exercitationem iste culpa neque tempore accusantium nobis asperiores dicta, sunt vitae architecto adipisci, enim minus esse atque. Ab maxime ad numquam praesentium, est repellendus possimus, nam et, enim, ea quibusdam. Libero unde sunt nihil quibusdam dolorum iste quasi blanditiis quod soluta voluptates illum eaque temporibus, mollitia molestias, illo ratione dolore velit commodi quae at natus possimus ab. Molestiae sint quisquam unde provident magni, reprehenderit pariatur at facilis ipsam illum error, dignissimos ad temporibus, incidunt doloremque? Reiciendis voluptates facilis, eius dolores ab inventore, ducimus veniam eligendi. Impedit nemo accusamus unde!
Our Projects
In order to make a MicroOrganizer, we needed to find a enzyme that could be split in two parts, and restore its function when the parts meet. We chose β-galactosidase gene, LacZ. β-galactosidase can be split into two parts β-galactosidase-α and β-galactosidase-β (Ullman et al. 1967). The functional enzyme consisting of one alpha and one beta part hydrolyses lactose into galactose and glucose. This process produces on some occasions allolactose which induces the Lac-operon. This connection between the enzyme product and the Lac operon provides the needed signalling mechanism. We have isolated some different autotransporters and have not yet decided which to use.
References
Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli. Ullmann A, Jacob F, Monod J J Mol Biol. 1967 Mar 14; 24(2):339-43.
