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Team:NCTU Formosa/Notebook/test4
From 2014.igem.org
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<font color="#FF0000"><span style="font-size:1cm;">7/2</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">7/2</span></font><br></p> | ||
<p> | <p> | ||
| - | <font color="yellow">Transformation of | + | <font color="yellow">Transformation of</font> ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1)</p> |
<p> | <p> | ||
<font color="yellow">Cultivation of</font> [B0032+BFP+J61048] Cr in LB broth (G2)</p> | <font color="yellow">Cultivation of</font> [B0032+BFP+J61048] Cr in LB broth (G2)</p> | ||
Revision as of 13:10, 14 October 2014
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7/1
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Digestion of [B0032] Ar
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/2
Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1)
Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)
7/3
Mini-prep of [B0032+BFP+J61048] and then digestion: (XP)
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/4
Digestion of [T7 promoter](SP) (G2)
Cultivation of [J23101] Ar (G3)
7/5
PCR of[B0032+K592100+J61048*11] Ar (G1)
Electrophoresis of PCR products of(G1)
Ligation of [J23101+B0032+PSB1C3*3] Cr (G1)
Electrophoresis of plasmid and digestion products of[T7promoter](SP),[B0031+BFP+J61048](XP)
Ligation of[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)
7/6
Transformation of the ligation products made at 7/5 (G2)
7/7
Transformation of [J23101+B0032*5] Cr (G1)
Cultivation of [B0032+K592100+J61048*2] Ar (G1)
Cultivation of [J23101] Ar (G3)
Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)
7/8
Mini-prep of [B0032+K592100+J61048*2] Ar and then digest of only one of two: (XP) (G1)
PCR of[T7+B0032+BFP+J61048] (G2)
Digestion of [J23101] Ar (ES) (G3)
7/9
Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)
7/10
Mini-prep of [B0032+BFP+J61048]Cr
Digestion of [B0032+FP+J61048]Cr( XP)
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/12
Digestion of [B0032+BFP+J612048](XP), [T7+B0032+BFP+J61048]Kr (XP) (G2)
7/14
Electrophoresis of [B0032+BFP+J61048]Cr,[T7+B0032+BFP+J61048]Kr (G2)
7/15
Cultivation of [B0032+BFP+J61048]Cr,[PT7+B0032+BFP+J61048]Kr E.coli in LB broth (G2)
7/16
Mini-prep of [B0032+BFP+J61048],[PT7+B0032+BFP+J61048]
Digestion of[B0032+BFP+J61048]Cr(XP),[PT7+B032+BFP+J61048]Kr(ES) (G2)
7/17
Electrophoresis of the plasmid and digestion products made yesterday (G2)
7/19
PCR of[J23101+B0032*12] Cr (G1)
Electrophoresis of PCR products of [B0032+K592100+J61048*2] Ar ,digestion product of [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
7/20
Digestion of [B0032+K592100+J61048] Ar (EP) (G1)
Digestion of [J23101] Ar (ES) (G3)
Ligation of [B0032+K592100+J61048] Cr (G1)
Ligation of [J23101+B0032+PSB1K3] Kr (G3)
PCR of [J23101+B0032*4] Cr (G1)
Cultivation of [J61048+B0032*4]Cr E.coli in LB broth (G1)
Electrophoresis of the PCR products and digestion products of [J23101] Ar (SP) and [B0032+K592100+J61048] Ar (EP) (G1)
7/21
Transformation of ligation product made yesterday (G1)
Transformation of ligation product of [J23101+B0032+PSB1K3] Kr (G3)
Ligation of [mGFP+J61048+PSB1K3] Kr,[mGFP+B0015+PSB1K3] Kr (G3)
7/23
Cultivation of [B0032+K592100+J61048] Cr E.coli in LB broth (G1)
Digestion of [J23101+B0032*3] Cr (EP) (G1)
Digestion of [B0032+K592100+J61048] Ar (EP) (G1)
Ligation of [J23101+B0032] Cr (G1)
Mini-prep of [B0032+K592100+J61048] Cr
Transformation of [B0032+K592100+J61048] Cr and [J23101+B0032] Ar (G1)
7/24
PCR of [B0032+K592100+J61048*12] Cr (G1)
Electrophoresis of digestion products of[J23101+B0032*3] Cr (EP) and the PCR products (G1)
Transformation of [J23101+B0032] Cr (G1)
Digestion of [BFP] Cr (ES), [B0015] Ar (XP), [J61048] Ar (XP), [B0032] Ar (XP), [BFP+B00315*2] Kr (XP) (G3)
7/26
Cultivation of [J23101+B0032*4] Cr E.coli in LB broth (G1)
Digestion of [J23101] Ar (SP), [B0032] Ar (XP) (G3)
7/27
Ligation of [J23101+B0032+PSB1C3] Cr (G3)
7/28
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Transformation of [J23101] Ar (G3)
PCR of [J23101+B0032+PSB1C3*6] Cr (G3)
7/29
Electrophoresis of the digestion products of[B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
Electrophoresis of the digestion product of [B0032] Ar (EP),[PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) and PCR product [J23101*6] Ar (G3)
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Digestion of [B0032] Ar (EP), [PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) (G3)
Mini-prep of [PT7+B0032+BFP+J61048] (G2)
PCR of [J23101*6] Ar (G3)
7/30
Electrophoresis of the digestion products of [B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
Electrophoresis of the plasmid and digestion products of [RBS],[B0032+BFP+J61048](XP),[PT7+B0032+BFP+J61048](XP) (G2)
7/31
Digestion of [B0032+K592100+J61048*2] Cr (XP) and [J23101] Ar (EP) (G1)
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Electrophoresis of the plasmid and digestion products of [B0032+K592100+J61048*2] Cr (XP) ,digestion products made in 7/7 and 7/30[B0032+K592100+J61048*2] Cr (XP) ; digestion product made in 7/31[B0032+K592100+J61048*2] Ar (XP) (G1)
