Team:NCTU Formosa/Notebook/test4

7/1

Ligation of [J23101+B0032+PSB1C3] Cr (G1)

Digestion of [B0032] Ar

Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/2

Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1)

Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)

7/3

Mini-prep of [B0032+BFP+J61048] and then digestion: (XP)

Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/4

Digestion of [T7 promoter](SP) (G2)

Cultivation of [J23101] Ar (G3)

7/5

PCR of [B0032+K592100+J61048*11] Ar (G1)

Electrophoresis of PCR products of(G1)

Ligation of [J23101+B0032+PSB1C3*3] Cr (G1)

Electrophoresis of plasmid and digestion products of[T7promoter](SP),[B0031+BFP+J61048](XP)

Ligation of [T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)

7/6

Transformation of the ligation products made at 7/5 (G2)

7/7

Transformation of [J23101+B0032*5] Cr (G1)

Cultivation of [B0032+K592100+J61048*2] Ar (G1)

Cultivation of [J23101] Ar (G3)

Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)

7/8

Mini-prep of [B0032+K592100+J61048*2] Ar and then digest of only one of two: (XP) (G1)

PCR of [T7+B0032+BFP+J61048] (G2)

Digestion of [J23101] Ar (ES) (G3)

7/9

Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)

7/10

Mini-prep of [B0032+BFP+J61048]Cr

Digestion of [B0032+FP+J61048]Cr( XP)

Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/12

Digestion of [B0032+BFP+J612048](XP), [T7+B0032+BFP+J61048]Kr (XP) (G2)

7/14

Electrophoresis of [B0032+BFP+J61048]Cr,[T7+B0032+BFP+J61048]Kr (G2)

7/15

Cultivation of [B0032+BFP+J61048]Cr,[PT7+B0032+BFP+J61048]Kr E.coli in LB broth (G2)

7/16

Mini-prep of [B0032+BFP+J61048],[PT7+B0032+BFP+J61048]

Digestion of [B0032+BFP+J61048]Cr(XP),[PT7+B032+BFP+J61048]Kr(ES) (G2)

7/17

Electrophoresis of the plasmid and digestion products made yesterday (G2)

7/19

PCR of [J23101+B0032*12] Cr (G1)

Electrophoresis of PCR products of [B0032+K592100+J61048*2] Ar ,digestion product of [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)

7/20

Digestion of [B0032+K592100+J61048] Ar (EP) (G1)

Digestion of [J23101] Ar (ES) (G3)

Ligation of [B0032+K592100+J61048] Cr (G1)

Ligation of [J23101+B0032+PSB1K3] Kr (G3)

PCR of [J23101+B0032*4] Cr (G1)

Cultivation of [J61048+B0032*4]Cr E.coli in LB broth (G1)

Electrophoresis of the PCR products and digestion products of [J23101] Ar (SP) and [B0032+K592100+J61048] Ar (EP) (G1)

7/21

Transformation of ligation product made yesterday (G1)

Transformation of ligation product of [J23101+B0032+PSB1K3] Kr (G3)

Ligation of [mGFP+J61048+PSB1K3] Kr,[mGFP+B0015+PSB1K3] Kr (G3)

7/23

Cultivation of [B0032+K592100+J61048] Cr E.coli in LB broth (G1)

Digestion of [J23101+B0032*3] Cr (EP) (G1)

Digestion of [B0032+K592100+J61048] Ar (EP) (G1)

Ligation of [J23101+B0032] Cr (G1)

Mini-prep of [B0032+K592100+J61048] Cr

Transformation of [B0032+K592100+J61048] Cr and [J23101+B0032] Ar (G1)

7/24

PCR of [B0032+K592100+J61048*12] Cr (G1)

Electrophoresis of digestion products of[J23101+B0032*3] Cr (EP) and the PCR products (G1)

Transformation of [J23101+B0032] Cr (G1)

Digestion of [BFP] Cr (ES), [B0015] Ar (XP), [J61048] Ar (XP), [B0032] Ar (XP), [BFP+B00315*2] Kr (XP) (G3)

7/26

Cultivation of [J23101+B0032*4] Cr E.coli in LB broth (G1)

Digestion of [J23101] Ar (SP), [B0032] Ar (XP) (G3)

7/27

Ligation of [J23101+B0032+PSB1C3] Cr (G3)

7/28

Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)

Transformation of [J23101] Ar (G3)

PCR of [J23101+B0032+PSB1C3*6] Cr (G3)

7/29

Electrophoresis of the digestion products of[B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)

Electrophoresis of the digestion product of [B0032] Ar (EP),[PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) and PCR product [J23101*6] Ar (G3)

Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)

Digestion of [B0032] Ar (EP), [PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) (G3)

Mini-prep of [PT7+B0032+BFP+J61048] (G2)

PCR of [J23101*6] Ar (G3)

7/30

Electrophoresis of the digestion products of [B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)

Electrophoresis of the plasmid and digestion products of [RBS],[B0032+BFP+J61048](XP),[PT7+B0032+BFP+J61048](XP) (G2)

7/31

Digestion of [B0032+K592100+J61048*2] Cr (XP) and [J23101] Ar (EP) (G1)

Ligation of [J23101+B0032+PSB1C3] Cr (G1)

Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)

Electrophoresis of the plasmid and digestion products of [B0032+K592100+J61048*2] Cr (XP) ,digestion products made in 7/7 and 7/30[B0032+K592100+J61048*2] Cr (XP) ; digestion product made in 7/31[B0032+K592100+J61048*2] Ar (XP) (G1)