Team:NCTU Formosa/Notebook/test3

From 2014.igem.org

(Difference between revisions)
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<p>
<p>
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6/2</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/2</span></font><br></p>
<p>
<p>
PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)</p>
PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)</p>
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Electrophoresis of  [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)</p>
Electrophoresis of  [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)</p>
<p>
<p>
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6/3</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/3</span></font><br></p>
<p>
<p>
PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5]  Kr (G2)</p>
PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5]  Kr (G2)</p>
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Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)</p>
Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)</p>
<p>
<p>
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6/5</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/5</span></font><br></p>
<p>
<p>
Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1)</p>
Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1)</p>
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Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
<p>
<p>
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6/6</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/6</span></font><br></p>
<p>
<p>
Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)</p>
Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)</p>
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Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
<p>
<p>
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6/7</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/7</span></font><br></p>
<p>
<p>
PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)</p>
PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)</p>
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<div  class="single note">
<div  class="single note">
<p>
<p>
-
6/8</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/8</span></font><br></p>
<p>
<p>
Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)</p>
Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)</p>
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Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr  (G2)</p>
Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr  (G2)</p>
<p>
<p>
-
6/9</p>
+
<font color="#FF0000"><span style="font-size:1cm;">6/9</span></font><br></p>
<p>
<p>
Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)</p>
Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)</p>
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Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES)  (G2)</p>
Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES)  (G2)</p>
<p>
<p>
-
6/10</p>
+
<font color="#FF0000"><span style="font-size:1cm;">6/10</span></font><br></p>
<p>
<p>
Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)</p>
Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)</p>
<p>
<p>
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6/11</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/11</span></font><br></p>
<p>
<p>
Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)</p>
Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)</p>
<p>
<p>
-
6/12</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/12</span></font><br></p>
<p>
<p>
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
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PCR: [Plac+B0032+PSB1K3*12] Kr (G3)</p>
PCR: [Plac+B0032+PSB1K3*12] Kr (G3)</p>
<p>
<p>
-
6/13</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/13</span></font><br></p>
<p>
<p>
Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p>
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Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)</p>
Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)</p>
<p>
<p>
-
6/14</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/14</span></font><br></p>
<p>
<p>
Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)</p>
Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)</p>
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<div  class="single note">
<div  class="single note">
<p>
<p>
-
6/15</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/15</span></font><br></p>
<p>
<p>
Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3)</p>
Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3)</p>
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<div  class="single note">
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<p>
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6/24</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/24</span></font><br></p>
<p>
<p>
Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1)</p>
Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1)</p>
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Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)</p>
Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)</p>
<p>
<p>
-
6/27</p>
+
<font color="#FF0000"><span style="font-size:1cm;">6/27</span></font><br></p>
<p>  
<p>  
Digestion of [J61048+J61048] Cr (XP) (G2)</p>
Digestion of [J61048+J61048] Cr (XP) (G2)</p>
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Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)</p>
Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)</p>
<p>
<p>
-
6/28</p>  
+
<font color="#FF0000"><span style="font-size:1cm;">6/28</span></font><br></p>  
<p>
<p>
Transformation of mini:[B0032+BFP+J61048] Cr (G2)</p>
Transformation of mini:[B0032+BFP+J61048] Cr (G2)</p>
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<div  class="single note">
<p>
<p>
-
6/29</p>
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<font color="#FF0000"><span style="font-size:1cm;">6/29</span></font><br></p>
<p>  
<p>  
PCR:[B0032+BFP+J61048]Cr</p>
PCR:[B0032+BFP+J61048]Cr</p>

Revision as of 11:41, 14 October 2014

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6/2

PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)

Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)

Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)

6/3

PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2)

Electrophoresis of PCR products (G2)

Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)

6/5

Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1)

Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2)

Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/6

Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)

Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2)

Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2)

Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/7

PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)

PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)


6/8

Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)

PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

6/9

Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)

Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2)

Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)

6/10

Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)

6/11

Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)

6/12

Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

PCR: [Plac+B0032+PSB1K3*12] Kr (G3)

6/13

Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)

6/14

Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)

Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)

PCR: [mGFP+B0015+PSB1K3*6] Kr


6/15

Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3)

Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)


6/24

Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1)

Ligation of [B0032+K592100+J61048] Ar (G1)

Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1)

Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)

6/27

Digestion of [J61048+J61048] Cr (XP) (G2)

Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)

6/28

Transformation of mini:[B0032+BFP+J61048] Cr (G2)


6/29

PCR:[B0032+BFP+J61048]Cr

Electrophoresis of the PCR products (G2)


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