Team:NCTU Formosa/Notebook/test3

From 2014.igem.org

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6/2

PCR of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)

Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)

Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)

6/3

PCR of [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2)

Electrophoresis of PCR products (G2)

Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)

6/5

Transformation of ligation product [K592100+J61048+PSBIK3] Kr (G1)

Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2)

Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/6

Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)

Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2)

Transformation of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2)

Transformation of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

6/7

PCR of [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)

PCR of [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)


6/8

Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)

PCR of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)

6/9

Transformation of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)

Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2)

Mini-prep of the plasmid and then digested the plasmid of [T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)

6/10

Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)

6/11

Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)

6/12

Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

PCR of [Plac+B0032+PSB1K3*12] Kr (G3)

6/13

Transformation of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)

Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)

6/14

Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)

Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)

PCR of [mGFP+B0015+PSB1K3*6] Kr


6/15

Mini-prep of [Plac+B0032+PSB1K3*4] Kr (G3)

Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)


6/24

Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1)

Ligation of [B0032+K592100+J61048] Ar (G1)

Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1)

Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)

6/27

Digestion of [J61048+J61048] Cr (XP) (G2)

Electrophoresis of [RBS+BFP]kr(ES)(mini),[T7polymerase](ES)(mini),[T7 promoter](mini)

6/28

Transformation of mini [B0032+BFP+J61048] Cr (G2)


6/29

PCR of [B0032+BFP+J61048]Cr

Electrophoresis of the PCR products (G2)


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