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Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs
From 2014.igem.org
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<td width="30%" id="KRXdeltadcuB"> <i>E.coli</i> KRX ΔdcuB::oprF</td> | <td width="30%" id="KRXdeltadcuB"> <i>E.coli</i> KRX ΔdcuB::oprF</td> | ||
<td width="40%">[F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF </td> | <td width="40%">[F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF </td> | ||
Revision as of 00:38, 14 October 2014
Strains
| Name | Properties / Genotype | BioBrick / Reference |
|---|---|---|
| General | ||
| Escherichia coli KRX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase | Promega |
| E. coli DH5α | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 | invitrogen |
| Module 1 | ||
| E.coli KRX ΔdcuB::oprF | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF | BBa_K1172507 |
| JW4084-1 | ΔdcuB749::kan | Baba et al., 2006 |
| JW0506-1 | ΔfdrA730::kan | Baba et al., 2006 |
| Actinobacillus succinogenes 130Z | wild type | Guettler et al., 1999 |
| Geobacter sulfurreducens PCA | wild type | Viulu et al., 2013 |
| Module 2 | ||
| E.coli BL21 | F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | NEB |
| Module 3 | ||
| JW1228-1 | ΔadhE748::kan | Baba et al., 2006 |
| Biosafety | ||
| E. coli KRX Δalr | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, Δalr | |
| E. coli KRX ΔdadX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX | |
| E. coli KRX Δalr ΔdadX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX, Δalr | |
| E. coli DH5α Δalr | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr | |
| E. coli DH5α ΔdadX | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 ΔdadX | |
| E. coli DH5α Δalr ΔdadX | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr ΔdadX | |
References
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Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
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Guettler, M. V., Rumler, D., Jain, M. K. (1999). Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing bacterium from the bovine rumen. Int.J.Syst.Bacteriol. 49 : 207-216 .
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Viulu, S., Nakamura, K., Kojima, A., Yoshiyasu, Y., Saitou, S., Takamizawa, K. (2013). Geobacter sulfurreducens subsp. ethanolicus, subsp. nov., an ethanol-utilizing dissimilatory Fe(III)-reducing bacterium from a lotus field. J Gen Appl Microbiol 59 ( 5 ): 325-34 .
Constructs
| Name | Properties | BioBrick / Reference |
|---|---|---|
| General | ||
| _ | ||
| Module 1 | ||
| _ | ||
| Module 2 | ||
| _ | ||
| Module 3 | ||
| --- | ||
| Biosafety | ||
| --- | ||
References
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paper
