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Team:Bielefeld-CeBiTec/Results/Biosafety
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Revision as of 20:02, 13 October 2014
Biosafety
Discussion you will find information about the execution of our experiments.
An important aspect of Synthetic Biology is to prevent the uncontrolled interaction between the genetically modified organismen and the environment and mankind. For this approach there are a lot of ideas to implement different Biosafety-Systems (Quelle) in synthetic Biology to prevent this interaction on one hand and one the other hand there are several studies dealing with the interaction of genetically modified bacteria and natural wild types, demonstrating in most cases that genetically modified bacteria does not influence the environment. Because the genetically modified bacteria are adapted to the excellent conditions of the laboratory, the natural bacteria will usually outlast these modified strains in nature due to their better adaptation to their environment. But there is always a risk remaining and no guarantee that there is really no interaction and that their release does not affect the equilibrium of the environment (Myhr et. al 1999).
So the most problematic factor is the transfection with synthetic or gentically modified DNA, particularly the antibiotic-resistance. A genetic exchange of the antibiotic-resistance is an active intervention in the enviroment. So the common factor and most problematic aspect of synthetic biology and its application is the antibiotic-resistance. Therefore we want to establish an antibiotic-free selection system, which can be used for molecular cloning as well as long-term plasmid stability.
Bilder Testreihe
https://static.igem.org/mediawiki/2014/7/7b/Bielefeld-CeBiTec_2014-10-13_Biosafety_Selection.png
So the most problematic factor is the transfection with synthetic or gentically modified DNA, particularly the antibiotic-resistance. A genetic exchange of the antibiotic-resistance is an active intervention in the enviroment. So the common factor and most problematic aspect of synthetic biology and its application is the antibiotic-resistance. Therefore we want to establish an antibiotic-free selection system, which can be used for molecular cloning as well as long-term plasmid stability.
Antibiotic-free Selection - Introduction
Bild D-Alanin Bild Zellwand
Transformation efficiency
Kloniengraphik
Bilder Testreihe
https://static.igem.org/mediawiki/2014/7/7b/Bielefeld-CeBiTec_2014-10-13_Biosafety_Selection.png
Transformation process
Verlauf Bilderreihe?
Molecular cloning without antibiotics
GFP
Long-term stability of the antiobiotic-free selection
Graphik
Remaining Challenges
MetC Reversion
Summary
References
