Team:UESTC-China/result
From 2014.igem.org
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<h1 class="SectionTitles" style="width:1140px; ">Plasmid Construction</h1><br/> | <h1 class="SectionTitles" style="width:1140px; ">Plasmid Construction</h1><br/> | ||
- | <p style="color:#1b1b1b;">We have successfully constructed 2 backbones, <em>piGEM001</em> and <em>piGEM002</em>. And we have verified them using digestion (Fig.1 | + | <p style="color:#1b1b1b;">We have successfully constructed 2 backbones, <em>piGEM001</em> and <em>piGEM002</em>. And we have verified them using digestion (Fig.1) and sequencing.</p> |
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- | <div align="center"><img style="width:300px;height:280px ;" src="https://static.igem.org/mediawiki/2014/ | + | <div align="center"><img style="width:300px;height:280px ;" src="https://static.igem.org/mediawiki/2014/c/cf/Rrfig1.JPG"> |
- | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"><strong>Fig.1</strong> | + | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"> |
- | Digestion the plasmid piGEM001 with HpaI and SpeI. | + | <strong>Fig.1</strong> |
+ | A. Digestion the plasmid piGEM001 with HpaI and SpeI. | ||
M: DNA molecular weight marker; | M: DNA molecular weight marker; | ||
1: plasmid piGEM001 and its digestion product | 1: plasmid piGEM001 and its digestion product | ||
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Digestion the plasmid piGEM002 with HpaI and DraIII. | Digestion the plasmid piGEM002 with HpaI and DraIII. | ||
M: DNA marker; | M: DNA marker; | ||
- | 1: plasmid piGEM002 and its digestion production | + | 1: plasmid piGEM002 and its digestion production |
</p> | </p> | ||
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<p style="color:#1b1b1b;">Then we have successfully constructed 6 monogene expression vectors, from vector <em>piGEM003</em> to vector <em>piGEM008</em>. And we have verified all of them using digestion and sequcencing. Here we only show the result of vector <em>piGEM005</em> (Fig.3). You can browse notebook for more results.</p> | <p style="color:#1b1b1b;">Then we have successfully constructed 6 monogene expression vectors, from vector <em>piGEM003</em> to vector <em>piGEM008</em>. And we have verified all of them using digestion and sequcencing. Here we only show the result of vector <em>piGEM005</em> (Fig.3). You can browse notebook for more results.</p> | ||
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Revision as of 12:55, 13 October 2014