Team:Aachen/Collaborations/Heidelberg

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= '''The project X-press''': Characterization of pSBX-vectors made by [[Team:Heidelberg|Team Heidelberg]] =
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= '''X-press''': Characterization of [[Team:Heidelberg|Team Heidelberg]]s pSBX-vectors =
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.

Revision as of 17:52, 12 October 2014

X-press: Characterization of Team Heidelbergs pSBX-vectors

The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.

In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.

The Team Heidelberg expression vectors

These are the tested constructs:

Plasmid Backbone Insert Length vector [bp] Length Insert [bp] Resistance
pSBX1A3-BBa_13507 pSBX1A3 (high copy) BBa_13507 3067 1226 50 mg/ml Ampicilin
pSBX1C3-BBa_13507 pSBX1C3 (high copy) BBa_13507 2982 1226 35 mg/ml Chloramphenicol
pSBX1K3-BBa_13507 pSBX1K3 (high copy) BBa_13507 3116 1226 50 mg/ml Kanamycin
pSBX1T3-BBa_13507 pSBX1T3 (high copy) BBa_13507 3322 1175 10 mg/ml Tetracyclin
pSBX4A5-BBa_13507 pSBX4A5 (low copy) BBa_13507 4307 1192 50 mg/ml Ampicilin
pSBX4C5-BBa_13507 pSBX4C5 (low copy) BBa_13507 4127 1186 15 mg/ml Chloramphenicol
pSBX4K5-BBa_13507 pSBX4K5 (low copy) BBa_13507 4331 1192 50 mg/ml Kanamycin
positive control: RFP selection marker with constant expression of mRFP 15 mg/ml Chloramphenicol



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