Revision as of 17:26, 12 October 2014

WatsOn

One part of our biosensor system Cellock Holmes are our agar chips containing our engineered sensing cells that fluoresce in the presence of the pathogen Pseudomonas aeruginosa. However, bacteria are small and the fluorescent signal cannot be seen with the naked eye. Thus the central question "What's on the chip?" remains. To answer this question we present our measurement device WatsOn.

WatsOn fulfills several tasks in our system:

incubate the sensing cells and the sampling chip
prevent escape of potentially sampled pathogens and our genetically engineered cells
illuminate the chip with the right excitation wavelength for GFP
take a photo of the chips
analyze the fluorescence signal
give feedback to the user about the presence or absence of P. aeruginosa

Measurarty

Measurements

Software Evaluation

Secret Ingredients

Arduino Sketchbook (.ino)

Build your own WatsOn

If you want to create your own WatsOn at first check the following list of necessary devices.

Table 1: Needed number of pieces, components and costs for building your own measurement device

number of pieces	components	costs [$]
1	[http://www.prolighting.de/Zubehoer/Farbfilter/Lee-Filter_HT/Lee-Filters_Musterheft_Designer_Edition_i174_3965_0.htm filter slides] (medium yellow 010, Double G&T 514)	5.17
1	[http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600 arduino UNO R3]	11.65
1	[http://www.dx.com/p/arduino-2-channel-relay-shield-module-red-144140 2-channel relay shield]	3.46
40	jumper-wire cable	2.99
1	[http://www.dx.com/p/2-54mm-1x40-pin-breakaway-straight-male-header-10-piece-pack-144191 40er male header (10-Piece Pack)]	2.72
1	[http://www.dx.com/p/jtron-2-54mm-40-pin-single-row-seat-single-row-female-header-black-10-pcs-278953 40er female header (10-Piece Pack)]	2.60
1	[http://www.dx.com/p/prototype-universal-printed-circuit-board-breadboard-brown-5-piece-pack-130926 circuit board]	2.99
1	[http://www.newark.com/pro-signal/rp006/audio-video-cable-hdmi-1m-black/dp/96T7446 HDMI cable]	1.87
1	[http://www.dx.com/p/hd-053-high-speed-usb-2-0-7-port-hub-black-174817 7 port USB hub]	6.71
1	[http://www.dx.com/p/dx-original-ultra-mini-usb-2-0-802-11n-b-g-150mbps-wi-fi-wlan-wireless-network-adapter-black-252716 WLAN USB stick]	5.35
1	USB mouse and keyboard	12.50
1	[http://corporate.evonik.com/en/products/pages/default.aspx case acrylic glass XT 6mm~0.5²]	50.65
1	spray paint for acrylic glass	6.54
1	[http://www.newark.com/raspberry-pi/raspberry-modb-512m/raspberry-pi-model-b-board/dp/68X0155 Raspberry Pi model B board]	35.00
1	[http://www.newark.com/raspberry-pi/rpi-camera-board/add-on-brd-camera-module-raspberry/dp/69W0689 Raspberry Pi camera module]	25.00
1	[http://www.pollin.de/shop/dt/NzUwOTc4OTk-/ 7” touchscreen display]	50.50
1	[http://www.dx.com/p/diy8-x-seven-segment-displays-module-for-arduino-595-driver-250813 8-segment display]	3.86
2	[http://www.dx.com/p/arduino-dht11-digital-temperature-humidity-sensor-138531 digital temperature sensor DHT-22]	7.50
1	aluminum block 100x100x15 mm	14.23
1	[http://www.dx.com/p/tec1-12706-semiconductor-thermoelectric-cooler-peltier-white-157283 Peltier heater 12V 60W]	4.49
1	power supply	32.89
6	[http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html superflux LED 480nm]	7.50
16	[http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html LED 450nm]	20.00
1	cupboard button	1.24
-	total	293.16

	Start building your own WatsOn by assembling the base plate, the sides and the interior wall.

Contact Disclaimer

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+| [[File:Aachen_Device_2_3.jpg|300px] [File:Aachen_Device_.3jpg|300px]] ||
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Team:Aachen/Project/Measurement Device

From 2014.igem.org