Team:Aachen/Collaborations/Heidelberg

From 2014.igem.org

(Difference between revisions)
(→The Team Heidelberg expression vectors)
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== The Team Heidelberg expression vectors ==
== The Team Heidelberg expression vectors ==
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These are the tested expression backbones:
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These are the tested constructs:
{| class="wikitable"
{| class="wikitable"
! Plasmid !! Backbone !! Insert !! Length vector !! Length Insert !! Resistance
! Plasmid !! Backbone !! Insert !! Length vector !! Length Insert !! Resistance
-
| pSBX1A3-BBa_13507 (high copy) || pSBX1A3 || BBa_13507 || 3067 bp || 1226 bp || 50 mg/ml Ampicilin
 
|-
|-
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| pSBX1C3-BBa_13507 (high copy) || pSBX1C3 || BBa_13507 || 2982 bp || 1226 bp || 35 mg/ml Chloramphenicol
+
| pSBX1A3-BBa_13507 || pSBX1A3 (high copy) || BBa_13507 || 3067 bp || 1226 bp || 50 mg/ml Ampicilin
|-
|-
 +
| pSBX1C3-BBa_13507 || pSBX1C3 (high copy) || BBa_13507 || 2982 bp || 1226 bp || 35 mg/ml Chloramphenicol
 +
|-
 +
| pSBX1K3-BBa_13507 || pSBX1K3 (high copy) || BBa_13507 || 3116 bp || 1226 bp || 50 mg/ml Kanamycin
 +
|-
 +
| pSBX1T3-BBa_13507 || pSBX1T3 (high copy) || BBa_13507 || 3322 bp || 1175 bp || 10 mg/ml Tetracyclin
 +
|-
 +
| pSBX4A5-BBa_13507 || pSBX4A5 (low copy) || BBa_13507 || 4307 bp || 1192 bp || 50 mg/ml Ampicilin
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|-
 +
| pSBX4C5-BBa_13507 || pSBX4C5 (low copy) || BBa_13507 || 4127 bp || 1186 bp || 15 mg/ml Chloramphenicol
 +
|-
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| pSBX4K5-BBa_13507 || pSBX4K5 (low copy) || BBa_13507 || 4331 bp || 1192 bp || 50 mg/ml Kanamycin
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|-
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| positive control: RFP selection marker with constant expressin of mRFP, 15 mg/ml Chloramphenicol
|}
|}
 +
 +
{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Revision as of 14:51, 12 October 2014

The project X-press: Characterization of pSBX-vectors made by Team Heidelberg

The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.

In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.

The Team Heidelberg expression vectors

These are the tested constructs:

Plasmid Backbone Insert Length vector Length Insert Resistance
pSBX1A3-BBa_13507 pSBX1A3 (high copy) BBa_13507 3067 bp 1226 bp 50 mg/ml Ampicilin
pSBX1C3-BBa_13507 pSBX1C3 (high copy) BBa_13507 2982 bp 1226 bp 35 mg/ml Chloramphenicol
pSBX1K3-BBa_13507 pSBX1K3 (high copy) BBa_13507 3116 bp 1226 bp 50 mg/ml Kanamycin
pSBX1T3-BBa_13507 pSBX1T3 (high copy) BBa_13507 3322 bp 1175 bp 10 mg/ml Tetracyclin
pSBX4A5-BBa_13507 pSBX4A5 (low copy) BBa_13507 4307 bp 1192 bp 50 mg/ml Ampicilin
pSBX4C5-BBa_13507 pSBX4C5 (low copy) BBa_13507 4127 bp 1186 bp 15 mg/ml Chloramphenicol
pSBX4K5-BBa_13507 pSBX4K5 (low copy) BBa_13507 4331 bp 1192 bp 50 mg/ml Kanamycin
positive control: RFP selection marker with constant expressin of mRFP, 15 mg/ml Chloramphenicol



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