Team:Caltech/Notebook

From 2014.igem.org

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Revision as of 04:44, 23 June 2014



WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

Apparently there's a calendar feature to take care of this too???

Week One

Tuesday, 6/17/14
  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies
 We got absolutely no results today!!!

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject
 We got absolutely no results today!!!

Thursday, 6/19/14
  • Formulated plasmid design for the response regulation subproject
  • PCR plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
 With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
 Colonies were found and picked after transformation and incubation.


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